Difference between revisions of "Make you own CRISPR-Kit"
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− | For the gathering at Schloss Solitude in Stuttgart in late 2016 we ordered the CRISP-Kit from The ODIN. The proposed experiment and materials are great. However we thought that a futuristic new technology could look a bit more fancy. So we did a creative workshop, speculative prototyping, design you own CRISPR-Kit how you would imagine a kit for a new technology should look like. Here are some results from the workshop: | + | == General Reflections on CRISPR Kits == |
+ | [[File:CRIPSR_german.jpg|thumb|200px]] | ||
+ | [[File:CRIPSR_tuetensuppe.png|thumb|200px]] | ||
+ | Seems these kits have cause a lot of recent discussions... | ||
+ | * [https://www.heise.de/newsticker/meldung/Biohacking-Bundesamt-warnt-vor-Do-It-Yourself-Kits-3616860.html Heise-News - Biohacking: Bundesamt warnt vor Do-It-Yourself-Kits] | ||
+ | * [http://www.bvl.bund.de/DE/06_Gentechnik/04_Fachmeldungen/2017/2017_01_25_DIY-Kits.html Especially the Germans seem not to like it so much :-)] | ||
+ | * [http://blogs.plos.org/synbio/2017/02/05/synbio-and-biosecurity/ This author though says ''"My fears about bioweapons and synthetic biology lie in the potential for misuse from state-actors rather than hobbyists and ill-equipped non-state terrorists."''] | ||
+ | * [http://www.prweb.com/releases/accessible/bio/prweb14053058.htm ... and other replies from the DIY kit-makers] | ||
+ | * [https://www.nzz.ch/wissenschaft/genetik-baukasten-gentechnik-gehoert-nicht-ins-kinderzimmer-ld.1296534 and NZZ late as usual "Warns.."] | ||
+ | * [https://www.bvl.bund.de/ZKBS/DE/03_Fokusthemen/DIY-Biologie/DIY-Biologie_node.html Zentrale Kommission für die Biologische Sicherheit "Do-it-yourself-Biologie - Gentechnik für jedermann?"] | ||
+ | |||
+ | |||
+ | So we picked it up again furing our Cheese making workshop [[Chrisper-Chäsli]], to be continued in Tokyo... | ||
+ | |||
+ | and it's always interesting to compare it to regulations in other places... for example Indonesia, see [[PRG Ikan Hobi - GMO fish geeking]] | ||
+ | |||
+ | ==CRISPR Kit Reflections at [[The Laboratory Solitude]] - Dec 2016== | ||
+ | |||
+ | For the [http://wlu18www30.webland.ch/wordpress/workshops/the-laboratory-solitude/ gathering at Schloss Solitude] in Stuttgart in late 2016 we ordered the CRISP-Kit from The ODIN. The proposed experiment and materials are great. However we thought that a futuristic new technology could look a bit more fancy. So we did a creative workshop, speculative prototyping, design you own CRISPR-Kit how you would imagine a kit for a new technology should look like. Here are some results from the workshop: | ||
Found a CRISP-Box at the Student Project house:<br> | Found a CRISP-Box at the Student Project house:<br> | ||
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And also the ordered ODIN box finally arrived: <br> | And also the ordered ODIN box finally arrived: <br> | ||
− | [[File: | + | [[File:ODINBox.jpg|400px]] |
This is the original Kit with some nice extra stuff, T-Shirt and Schnapps-Glässli:<br> | This is the original Kit with some nice extra stuff, T-Shirt and Schnapps-Glässli:<br> | ||
Line 11: | Line 29: | ||
<br> | <br> | ||
<br> | <br> | ||
− | [[File:CRISPRWorkshop.jpg|500px]] | + | Let's start a workshop.<br> |
+ | [[File:CRISPRWorkshop.jpg|500px]]<br> | ||
<br> | <br> | ||
+ | Some hacked CRISPR Kits:<br> | ||
[[File:Gold_CRISPR_Kit.jpg|400px]]<br> | [[File:Gold_CRISPR_Kit.jpg|400px]]<br> | ||
+ | Gold-CRISPR Kit<br><br> | ||
[[File:CRHIPSTER_Kit.jpg|400px]]<br> | [[File:CRHIPSTER_Kit.jpg|400px]]<br> | ||
− | [[File:CRISPRKit1.jpg|400px]]<br> | + | The CRIHIPPSTER-Kit<br><br> |
− | + | [[File:CRISPRKit1.jpg|400px]]<br><br> | |
+ | <br> | ||
<br> | <br> | ||
+ | Tools and Pipettes:<br> | ||
[[File:Pipettes.jpg|300px]]<br> | [[File:Pipettes.jpg|300px]]<br> | ||
[[File:PipetteAndCRISPR.jpg|300px]]<br> | [[File:PipetteAndCRISPR.jpg|300px]]<br> | ||
− | [[File:MicroMedia.jpg| | + | [[File:MicroMedia.jpg|200px]]<br> |
− | [[File:PipettesAndPlasmids.jpg| | + | Pipettes with Plasmids:<br> |
− | [[File: | + | [[File:PipettesAndPlasmids.jpg|300px]]<br> |
+ | And special CRISPR petri dishes:<br> | ||
+ | [[File:CRISPR_Petris.jpg|400px]] | ||
+ | <br> | ||
+ | And some newly designed CRISPR organisms:<br> | ||
[[File:CRISPRS1.jpg|400px]]<br> | [[File:CRISPRS1.jpg|400px]]<br> | ||
[[File:CRISPRS2.jpg|400px]]<br> | [[File:CRISPRS2.jpg|400px]]<br> | ||
− | [[File:CRISPR3.jpg|400px]]<br> | + | [[File:CRISPR3.jpg|200px]]<br> |
+ | <br> | ||
+ | CRISPR him self:<br> | ||
+ | [[File:TheCRISPR.jpg|400px]]<br> | ||
+ | <br> | ||
+ | Josiha Zayner from The Open Discovery Institute (ODIN) calling in. <br> | ||
+ | [[File:Josiha.jpg|500px]]<br> | ||
+ | <br> | ||
<br> | <br> | ||
− | [[File: | + | The DIY hacker CRISPR Cas9 Kit:<br> |
+ | [[File:CRISPR_Kit2.jpg|550px]] | ||
+ | [[Category:Wetware]][[Category:Molecular]] |
Latest revision as of 12:51, 2 December 2017
General Reflections on CRISPR Kits
Seems these kits have cause a lot of recent discussions...
- Heise-News - Biohacking: Bundesamt warnt vor Do-It-Yourself-Kits
- Especially the Germans seem not to like it so much :-)
- This author though says "My fears about bioweapons and synthetic biology lie in the potential for misuse from state-actors rather than hobbyists and ill-equipped non-state terrorists."
- ... and other replies from the DIY kit-makers
- and NZZ late as usual "Warns.."
- Zentrale Kommission für die Biologische Sicherheit "Do-it-yourself-Biologie - Gentechnik für jedermann?"
So we picked it up again furing our Cheese making workshop Chrisper-Chäsli, to be continued in Tokyo...
and it's always interesting to compare it to regulations in other places... for example Indonesia, see PRG Ikan Hobi - GMO fish geeking
CRISPR Kit Reflections at The Laboratory Solitude - Dec 2016
For the gathering at Schloss Solitude in Stuttgart in late 2016 we ordered the CRISP-Kit from The ODIN. The proposed experiment and materials are great. However we thought that a futuristic new technology could look a bit more fancy. So we did a creative workshop, speculative prototyping, design you own CRISPR-Kit how you would imagine a kit for a new technology should look like. Here are some results from the workshop:
Found a CRISP-Box at the Student Project house:
And also the ordered ODIN box finally arrived:
This is the original Kit with some nice extra stuff, T-Shirt and Schnapps-Glässli:
Let's start a workshop.
Some hacked CRISPR Kits:
Gold-CRISPR Kit
The CRIHIPPSTER-Kit
Tools and Pipettes:
Pipettes with Plasmids:
And special CRISPR petri dishes:
And some newly designed CRISPR organisms:
CRISPR him self:
Josiha Zayner from The Open Discovery Institute (ODIN) calling in.
The DIY hacker CRISPR Cas9 Kit: