Bio Lab Infrastructure

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Art sci lab.jpg

How to start a DIWO art-sci bio lab?

If you can't build your lab, you don't own the lab

See a version from Marc Dusseiller aka dusjagr, Workshopologist and from Urs Gaudenz, Dipl. Ing. Mikrotechnik

See an old version of the slides

People not Infrastructure

  • Lab Making | The Book: HackteriaLab 2014 - Yogyakarta, by Urs Gaudenz, Sachiko Hirosue

Instructions on lab-making

"Making a Laboratory is both a spontaneous activity and a slow labor of love."

A Lab is a place to labor (not only of love), experiment, research and share - there is no labor, experiment, research or sharing without the people. A Lab is not a museum for journalists, but a place made by a person for other people Start a small Lab , put it in a box, suitcase, backpack, picnic basket and take it with you. A Mobile Lab it actually allows you to go to people, and not wait for the others to come.

Now also on Medium, revised edition.

Lab-luggages.jpg

  • Lab Infrastructure / Equipment

Experiences from setting up the BioTehna Lab Infrastructure. People not equipment. 20% Science 80% Rock'n'Roll.

Materials list for electronic prototyping from ali-express: here

Thoughts about Infrastructural Hospitality

People putting boxes to make people feel comfortable... like someone running a restaurant, make sure your people are well fed and feel comfortable.

Infrastructure

BioLab Equipment

image # what notes
BT hotplate.jpg 1 small hotplate
BT mixer.jpg 1 Mixer
BT balance.jpg 1 small balance 0.02g - 1000g
BT hotdog machine.jpg 1 HotDog machine it's like a small cooker/pot
BT grinder.jpg 1 Coffee grinder thanks bordul :-)
BT DIY micrscope.jpg 1 DIY microscope PS3eye made by tamberg, used by many
BT DIY micrscopy-kit.jpg 2 DIY Microscopy kits made by gaudi, Laser-cut microscopy stages
BT DIY spectroscope.jpg 1 DIY Spectroscope PS3eye made by Bengt, check public laboratory
BT mycelium scanner.jpg 1 scanner with embeddedincubator made by bostjan et al at NanoSmano 3

BioLab stuff

image # what notes
BT glassware.jpg 4 x Erlenmayer 0 x beakers 1 x 100ml graded cylinder
BT micropipette.jpg 1 check online free samples or chinese stuff
BT pots.jpg 2 small cooking pots we need a steampot for sterilization!
BT plasticware.jpg var. kitchen plastic ware many tupperware
BT DIY UV-drawer.jpg 1 x UV light bulp (black light) 1 x 254nm / 366nm tube 4 x UV-LED's
BT DIY gel electro.jpg 1 DIY gel-box var. Food colours 1 pack of Agar Agar we need more agar!

General Technical Equipment

image # what notes
BT soldering irons.jpg 6 Soldering Irons some of them with swiss plug...
BT lamps.jpg 3 Table lamps maybe get 3 more?
BT Power Supply.jpg 1 Power Supply 15V, 2A maybe get a stronger one, up to 80V
BT dremel.jpg 1 Cordless Dremel and toolsl belongs to dusjagr
BT labelling.jpg 1 Dymo LetraTag we need green ammo, from kniigica next to nama

Shared Tools for Hacking Mobile drawers

RAKO boxes labelled with Hackteria / Kapelica
image Stuff thing 1 thing 2 thing 3 thing 4 thing 5
BT Tools pliers.jpg 3 Table lamps maybe get 3 more?
BT Eksperiment.jpg 5 x breadboards 2 x multimeters crocodile cables Jumper wires
BT glues.jpg 2 x large glue gun (+ammo) 1 x small glue gun (+ammo) 1 x epoxy rubber bands cable binders get more small glue guns and loads of ammo
BT energija batteries.jpg 9V blocks 30 AA Batteries 20 AAA Batteries various power supplies 5 - 15V
Error creating thumbnail: File missing
4 x X-Mini active speakers 4-5 x random speakers some cables
BT helping hands.jpg 5 x Helping hands 2 x Unsoldering tools what else?

Electronics and Parts

RAKO boxes labelled with Hackteria / Kapelica
image Class Group 1 Group 2 Group 3 Group 4 Group 5
BT Electronics1 parts.jpg Chips 10 attiny 85 20 4093 various
BT uinos.jpg 4 x arduinos Table lamps get 10 large USB cables, and more arduinos
BT chips.jpg Chips 10 attiny 85 20 4093 various
BT mignon gamekit.jpg 2x Mignon Gamekit 3 x Programmers see info on [1]

Boxes

RAKO boxes labelled with Hackteria / Kapelica
image content content content Content notes
Box soldering.jpg 5 x Soldering Irons 1 x Solder wire 5 x sponges anything else?
Box electric.jpg 2 x Table lamps 2 x Power Plugs 3 x 220V Timers 3 x remote control 220V Timers
Box BioCyberKidzz.jpg 1 loads of fun stuff see BioCyberKidzz

Wishlist

Lab Gear

- trash bin for separating garbage
- incubator
- fridge with freezer
- additional shelves
- DIY PCR
- couple of shelves
- centrifuge
- OD meter

Stuff

Where to get stuff (in Ljubljana)

Organisms

http://lgcstandards-atcc.org/Default.aspx?base

http://www.ccap.ac.uk/

Chemicals

Slovenian distributors of lab chemicals, including enzymes and stuff

http://www.majbert.com/

http://www.kemomed.si/

http://www.omega.si/

http://www.kefo.si/

Everything you ever wanted to know about agar http://www.fao.org/docrep/X5822E/x5822e03.htm

Labware

http://www.tehtnica.si/

http://www.sanolabor.si/

http://market.mikro-polo.si/

http://dx.com/p/mg16129-c-5-led-auxiliary-clip-magnifier-black-white-eu-plug-110-240v-3-x-aa-193552

Tools

Materials

Electronics

http://www.hte.si/

http://www.conrad.si/ (supposedly too expensive)

http://dx.com/ order gadgets and electronics from china/HK

A/V equipment

Helpful local institutions, companies and other people we have had pleasant interactions with in the past

http://www.algen.si/

http://ibk.mf.uni-lj.si/defaultslo.html

http://www.arhiv.mop.gov.si/si/o_ministrstvu/organiziranost/direktorat_za_okolje/

Other links on how to start a DIWO BioHackArt lab

From the Waag Do-It-Together biolab check their File:Waag wishlist.pdf

Using eBay to set up a molecular biology lab: costs less than $1000!

exerpts from the DIYbio maillist assembled on openWetWare

Instructable how to set up a home chemistry lab

makezine, setting up a home science lab part 1 part 2, part 3

From IndieBiotech aka Cathal Garvey not much on it, but a start.

more of Cathal's protocolls on github

Good Microbiological Practice

http://www.lbl.gov/ehs/biosafety/manual/html/AppxD.shtml

Next level shit... how to get BSL1