Difference between revisions of "WetWare"
(Created page with "WetWare Shopping<br> <br> <br> <br> Chemie gut und günsitg von ebay:<br> http://stores.ebay.de/s3chemicals/<br> <br> The ODIN - The Open Discovery Institute - ebe:<br> http:/...") |
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Agarose and Tubes from ebay:<br> | Agarose and Tubes from ebay:<br> | ||
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br> | http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br> | ||
+ | <br> | ||
+ | WetWare Shopping<br> | ||
+ | <br> | ||
+ | For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:<br> | ||
+ | If you know a good store that you can recommend, please add it to the list.<br> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | Chemie gut und günsitg von ebay:<br> | ||
+ | http://stores.ebay.de/s3chemicals/<br> | ||
+ | <br> | ||
+ | The ODIN - The Open Discovery Institute - ebe:<br> | ||
+ | http://www.the-odin.com/<br> | ||
+ | <br> | ||
+ | Good quality TAQ and home brewing of biomaterials:<br> | ||
+ | https://www.geneandcell.com/<br> | ||
+ | <br> | ||
+ | Agarose and Tubes from ebay:<br> | ||
+ | http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48<br> | ||
+ | <br> | ||
+ | <br> | ||
+ | Poll on FaceBook by Alex Kelly:<br> | ||
+ | "Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":<br> | ||
+ | <br> | ||
+ | - "Sucrose and amino acids from sigma, sent to US residential adress for a legit company - but they insisted on an official lab"<br> | ||
+ | - Chloral hydrate<br> | ||
+ | - beakers<br> | ||
+ | - Methylamine<br> | ||
+ | - Love<br> | ||
+ | - Temperature regulators / thermostats. Julabo or Huber. No second hand units available in Australia<br> | ||
+ | - Believe it or not, pure ethanol.<br> | ||
+ | - Dichloromethane<br> | ||
+ | - The common inorganic acids (e.g. conc H2SO4)<br> | ||
+ | - Lithium acetate<br> | ||
+ | - Methacrylic anhydride<br> | ||
+ | - PEG in different molecular weights, PEG in 8000 and 3350<br> | ||
+ | - Glacial HOAc<br> | ||
+ | - Acetone AR<br> | ||
+ | - 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)<br> | ||
+ | - NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)<br> | ||
+ | - A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.<br> | ||
+ | - HCl<br> | ||
+ | - H2SO4<br> | ||
+ | - Etehanol<br> | ||
+ | - CaCl2<br> | ||
+ | - MgCl2<br> | ||
+ | - Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever<br> | ||
+ | - EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)<br> | ||
+ | - Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris<br> | ||
+ | - Glycerol and DMSO<br> | ||
+ | - Pure NaCl<br> | ||
+ | - Agarose<br> | ||
+ | - Agar<br> | ||
+ | - Common media such as LB as well as individual components such as yeast extract and peptone etc.<br> | ||
+ | - Premix common buffers such as TAE<br> | ||
+ | - Common antibiotics e.g. ampicillin<br> | ||
+ | - Dextrose<br> | ||
+ | - L-Arabinose<br> | ||
+ | - Organic solvents that don't leave residue when evaporated<br> | ||
+ | - Ion-exchange resins for DNA workup, like Chelex sort of stuff.<br> | ||
+ | - Chloroform and phenol.<br> | ||
+ | - Isoamyl alcohol<br> | ||
+ | - PCRMastermix<br> | ||
+ | - petridish<br> | ||
+ | - Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.<br> | ||
+ | - Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.<br> | ||
+ | - strains of mice<br> | ||
+ | - Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)<br> | ||
+ | - monopotassium phosphate and dipotassium phosphate<br> | ||
+ | <br> | ||
+ | <br> | ||
+ | Thanks to everyone who helped compile this list...<br> | ||
+ | "Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)<br> | ||
<br> | <br> |
Revision as of 09:33, 11 April 2017
WetWare Shopping
Chemie gut und günsitg von ebay:
http://stores.ebay.de/s3chemicals/
The ODIN - The Open Discovery Institute - ebe:
http://www.the-odin.com/
Good quality TAQ and home brewing of biomaterials:
https://www.geneandcell.com/
Agarose and Tubes from ebay:
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48
WetWare Shopping
For independent scientists or artists doing DIYBio it is not always easy to get the biomaterials and wet ware easily. Here is a list of shops that are hacker-friendly and cheap:
If you know a good store that you can recommend, please add it to the list.
Chemie gut und günsitg von ebay:
http://stores.ebay.de/s3chemicals/
The ODIN - The Open Discovery Institute - ebe:
http://www.the-odin.com/
Good quality TAQ and home brewing of biomaterials:
https://www.geneandcell.com/
Agarose and Tubes from ebay:
http://stores.ebay.com/greenbioresearch/_i.html?rt=nc&_pgn=1&_ipg=48
Poll on FaceBook by Alex Kelly:
"Sunday morning question: What basic equipment/reagent/material have you struggled to obtain on the open consumer market?":
- "Sucrose and amino acids from sigma, sent to US residential adress for a legit company - but they insisted on an official lab"
- Chloral hydrate
- beakers
- Methylamine
- Love
- Temperature regulators / thermostats. Julabo or Huber. No second hand units available in Australia
- Believe it or not, pure ethanol.
- Dichloromethane
- The common inorganic acids (e.g. conc H2SO4)
- Lithium acetate
- Methacrylic anhydride
- PEG in different molecular weights, PEG in 8000 and 3350
- Glacial HOAc
- Acetone AR
- 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Is a photoinitiator in case you're wondering.)
- NaOH solid (prills/pellets/whatever) (Must be AR, if I wanted Bunnings-grade I'd just buy it.)
- A lot of the acetate salts (and acid) tend to pop up a lot in molecular biology and biochemistry in buffers etc.
- HCl
- H2SO4
- Etehanol
- CaCl2
- MgCl2
- Cost-effective DNA dyes such as ethidium / GelRed / SYBR / whatever
- EDTA disodium dihydrate (sometimes people specify EDTA without specifying which salt and which hydrate, which is a pain because you don't specifically know what the counter ion is, or the molecular weight.)
- Glycerol, DMSO, MgCl2, CaCl2, EDTA, Tris
- Glycerol and DMSO
- Pure NaCl
- Agarose
- Agar
- Common media such as LB as well as individual components such as yeast extract and peptone etc.
- Premix common buffers such as TAE
- Common antibiotics e.g. ampicillin
- Dextrose
- L-Arabinose
- Organic solvents that don't leave residue when evaporated
- Ion-exchange resins for DNA workup, like Chelex sort of stuff.
- Chloroform and phenol.
- Isoamyl alcohol
- PCRMastermix
- petridish
- Schott bottles (or clones) in a spectrum of sizes including the little 100ml ones.
- Stock microorganisms. Nothing dangerous but the common risk group 1 stuff. Like stock of Saccharomyces and K12.
- strains of mice
- Stock plasmids (e.g. common expression vectors, scaffolds, Cas9 templates)
- monopotassium phosphate and dipotassium phosphate
Thanks to everyone who helped compile this list...
"Perhaps some of us should start a business selling scientific supplies, reagents etc marketed towards citizen scientist, hackers etc.. Could sell cheap kits for open source hardware too".. (Leo Mason)