Week Three
Contents
May 30th
All DNA pictures have been uploaded. Take your pick here
1) Extracting DNA from Banana: <<Read here
2) Exracting DNA from Saliva:
Images of our DNA we extracted from saliva:
Neha's DNA
Krupakar's DNA
Dhruv's DNA
Sandeep's DNA
May 31st
Some interesting articles:
http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology
http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html
An interesting work:
http://www.edgarlissel.de/data/mnemosyne_II_01e.html (The photosensitive stripes are made of bacteria)
June 3rd
Registry of Parts Exercise
Sanya: Bacteria that indicates rise in and regulates body temperature.Media:Light.jpg
June 5th
Beginning laboratory procedures:
1) Bacterial Transformation : The Process | The Bacterial Transformation Gallery
2) Preparing the Plasmid (minprep)
3) Digesting the DNA
4) Running the gel (Gel Electrophoresis)
5) Elution
June 8th
A Brief Description of What We Will Do
The Plasmid preparation process | The Plasmid Preparation Miniprep Gallery
A Brief of what we did:
The plasmids that contained the pBAD and Lysis genes that were prepared were extracted from the bacteria cultures. The unwanted protein around the DNA was then removed. After that the DNA was cleansed of any remaining salt. The plasmids were then cut at certain points such that only the required gene could be extracted. These cut parts were prepared for gel electrophoresis.
