Difference between revisions of "Week Three"

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(Created page with ' === May 30th === All DNA pictures have been uploaded. Take your pick here 1) '''Extracting DNA from Banana:''' <<Read here 2) '''Exracting DNA from...')
 
 
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>=== May 30th ===
  
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=== May 30th ===
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'''We extracted DNA from human saliva and banana in our semi-functional, backyard science lab!
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It was sort of like a pre-introduction to science which we did ourselves!'''
  
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All DNA pictures have been uploaded. Take your pick [[Special:ListFiles|here]]
 
 
   
 
   
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1) '''Extracting DNA from Banana:''' <<[[Read here]]
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1) '''Extracting DNA from Banana:''' &lt;&lt;[[Read here]]
  
 
2) '''Exracting DNA from Saliva:'''
 
2) '''Exracting DNA from Saliva:'''
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'''Images of our DNA we extracted from saliva:'''
 
'''Images of our DNA we extracted from saliva:'''
  
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<gallery>
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&lt;gallery&gt;
 
File:NehasDNA.JPG|Neha's DNA
 
File:NehasDNA.JPG|Neha's DNA
 
File:KrupakarsDNA.JPG|Krupakar's DNA
 
File:KrupakarsDNA.JPG|Krupakar's DNA
 
File:DhruvsDNA.JPG|Dhruv's DNA
 
File:DhruvsDNA.JPG|Dhruv's DNA
 
File:SandeepsDNA.JPG|Sandeep's DNA
 
File:SandeepsDNA.JPG|Sandeep's DNA
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</gallery>
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&lt;/gallery&gt;
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*All DNA pictures have been uploaded. Take your pick [[Special:ListFiles|here]]
 +
 
  
 
===May 31st===
 
===May 31st===
  
 
Some interesting articles:
 
Some interesting articles:
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'''The Promise and Perils of Synthetic Biology:'''
  
 
http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology
 
http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology
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'''Molecular Synthetic Biology:'''
  
 
http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html  
 
http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html  
  
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An interesting work:
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'''The photosensitive stripes are made of bacteria:'''
  
 
http://www.edgarlissel.de/data/mnemosyne_II_01e.html
 
http://www.edgarlissel.de/data/mnemosyne_II_01e.html
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(The photosensitive stripes are made of bacteria)
 
  
 
===June 3rd===
 
===June 3rd===
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'''''Registry of Parts Exercise'''''
 
  
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Sanya: Bacteria that indicates rise in and regulates body temperature.[[Media:Light.jpg]]
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'''The Registry of Parts Exercise'''
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To research a feasible idea among the various ideas that came up during the brainstorming session and present a dummy procedure on how to achieve the result on the basis of the scientific knowledge acquired in the two and a half weeks into iGEM.
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 +
Sanya:
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Bacteria that indicates rise in and regulates body temperature.[[Media:Light.jpg]]
  
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[[Upasana]]
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[[Upasana]]: Temperature regulating bacteria
  
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[[Neha's Bacterial Jewellery]]
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All That Glitters: [[Neha's Bacterial Jewelery]]
  
 
===June 5th===
 
===June 5th===
  
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'''Beginning laboratory procedures:'''
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'''Beginning laboratory procedures:''' Introducing ourselves to understand working in a professional laboratory.
  
 
1) [[Bacterial Transformation : The Process]] | [[The Bacterial Transformation Gallery]]
 
1) [[Bacterial Transformation : The Process]] | [[The Bacterial Transformation Gallery]]
  
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2) Preparing the Plasmid (minprep)
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2) Preparing the Plasmid (miniprep)
  
 
3) Digesting the DNA
 
3) Digesting the DNA
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5) Elution
 
5) Elution
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===June 8th===
 
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'''''[[A Brief Description of What We Will Do]]'''''
 
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[[Preparing the pBAD and Lysis plasmids for gel electrophoresis|The Plasmid preparation process]] | [[The Plasmid Preparation Miniprep Gallery]]
 
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''' A Brief of what we did: ''' <br/>
 
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The plasmids that contained the pBAD and Lysis genes that were prepared were extracted from the bacteria cultures. The unwanted protein around the DNA was then removed. After that the DNA was cleansed of any remaining salt. The plasmids were then cut at certain points such that only the required gene could be extracted. These cut parts were prepared for gel electrophoresis.
 

Latest revision as of 21:33, 11 December 2010

>=== May 30th ===

We extracted DNA from human saliva and banana in our semi-functional, backyard science lab!

It was sort of like a pre-introduction to science which we did ourselves!


1) Extracting DNA from Banana: <<Read here

2) Exracting DNA from Saliva:

Images of our DNA we extracted from saliva:

<gallery> File:NehasDNA.JPG|Neha's DNA File:KrupakarsDNA.JPG|Krupakar's DNA File:DhruvsDNA.JPG|Dhruv's DNA File:SandeepsDNA.JPG|Sandeep's DNA </gallery>

  • All DNA pictures have been uploaded. Take your pick here


May 31st

Some interesting articles:

The Promise and Perils of Synthetic Biology:

http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology

Molecular Synthetic Biology:

http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html

The photosensitive stripes are made of bacteria:

http://www.edgarlissel.de/data/mnemosyne_II_01e.html

June 3rd

The Registry of Parts Exercise

To research a feasible idea among the various ideas that came up during the brainstorming session and present a dummy procedure on how to achieve the result on the basis of the scientific knowledge acquired in the two and a half weeks into iGEM.

Sanya: Bacteria that indicates rise in and regulates body temperature.Media:Light.jpg

Upasana: Temperature regulating bacteria

All That Glitters: Neha's Bacterial Jewelery

June 5th

Beginning laboratory procedures: Introducing ourselves to understand working in a professional laboratory.

1) Bacterial Transformation : The Process | The Bacterial Transformation Gallery

2) Preparing the Plasmid (miniprep)

3) Digesting the DNA

4) Running the gel (Gel Electrophoresis)

5) Elution

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