Difference between revisions of "Week Four"

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(June 8th- 17th)
(June 8th- 17th)
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'''[[Step 2 - Transforming competent cells.]]'''
'''[[Step 2 - Transforming competent cells.]]'''
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.''
'''Step 3 - Picking a single colony.'''
'''Step 3 - Picking a single colony.'''
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'''Step 7 - [[Gel Electrophoresis]]'''
'''Step 7 - [[Gel Electrophoresis]]'''
'''Step 8-[[Ligation]]'''
'''Step 8- [[Ligation]]'''
===June 9th===
===June 9th===

Revision as of 07:19, 17 June 2009

June 8th- 17th

Our Step-wise Process:

Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells.

Step 3 - Picking a single colony.

Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA

Step 7 - Gel Electrophoresis

Step 8- Ligation

June 9th

The June 9th Image Gallery

Images of the various results attained:

For June 15th:

These bands will be kept overnight in 37 degrees Celsius.

Then these will be run through a preparative gel which takes about 3 hours.

The next step is to elute the released products which wold take 1.5 hours.

CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.

This enzyme will then be inactivated at 65 degree C.

This vector needs to be purified using column or chloroform