Difference between revisions of "Week Four"

From Hackteria Wiki
Jump to: navigation, search
(June 8th)
(June 9th)
Line 13: Line 13:
'''For June 14th:'''
*(Lysis= K112808)
pBAD- promoter
Lysis-big construct- gene for cell lysis
Gel image of released pBAD(I0500), after digestion with EcoRI+PstI:
Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.
pBAD is digested with EcoRI +SPeI
Lysis is digested with EcoRI + XbaI
A small interlink sequence between E and X will break open and spread as one band
'''For June 15th:'''
'''For June 15th:'''

Revision as of 18:50, 16 June 2009

June 8th- 17th

Our Step-wise Process

June 9th

The June 9th Image Gallery

Images of the various results attained:

For June 15th:

These bands will be kept overnight in 37 degrees Celsius.

Then these will be run through a preparative gel which takes about 3 hours.

The next step is to elute the released products which wold take 1.5 hours.

CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.

This enzyme will then be inactivated at 65 degree C.

This vector needs to be purified using column or chloroform