Difference between revisions of "Week Four"
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===June 8th- 17th=== | ===June 8th- 17th=== | ||
− | Our Step-wise Process: | + | '''What are we trying to do?''' |
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+ | We want the bacteria to lyse, to kill itself from the inside. | ||
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+ | Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a '''promoter''' | ||
+ | |||
+ | There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. | ||
+ | Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme. | ||
+ | |||
+ | '''Our Step-wise Process:''' | ||
+ | |||
+ | How we tried to do this: | ||
'''[[Step 1 - Taking dry DNA from wells]]''' | '''[[Step 1 - Taking dry DNA from wells]]''' | ||
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'''Step 8- [[Ligation]]''' | '''Step 8- [[Ligation]]''' | ||
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+ | '''Step 9- [[Transformation and Inoculation]]''' | ||
===June 9th=== | ===June 9th=== | ||
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<gallery> | <gallery> | ||
− | File:Gelimage090609.png | + | File:Gelimage090609.png|Agarose gel electrophoresis image of digested K112808 |
− | File:Not working.jpg | + | File:Not working.jpg|Failure 1 |
− | File:PBAD-insert f.png | + | File:PBAD-insert f.png| The pBAD Insert |
+ | File:Failed Ligation.png| The Failed Ligation Attempt | ||
</gallery> | </gallery> | ||
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Latest revision as of 06:56, 26 June 2009
June 8th- 17th
What are we trying to do?
We want the bacteria to lyse, to kill itself from the inside.
Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter
There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.
Our Step-wise Process:
How we tried to do this:
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells
Step 3 - Picking a single colony.
Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Gel Electrophoresis
Step 8- Ligation
Step 9- Transformation and Inoculation
June 9th
Images of the various results attained: