Difference between revisions of "Samrajni Patil"
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Can't wait for Monday!:)
Can't wait for Monday!:)
Revision as of 18:49, 16 June 2010
To read special entries click here > The Specials
10th May 2010
Honestly all I knew about this competition was that it had something to do with biology and it was the memory of how much I used to love biology back in the tenth grade that motivated me to take it up. Also, there's a bit of a science fiction fan inside me!
We started work today. The majority of us being purely Art students came with the little knowledge that high school biology provides. We learned that we have missed out on a lot of details and that there is a sea of information out there that we need to know in order to fully and effectively exercise our creative skills.
Although I love biology I realised that I remember very little of what I had been taught, so I asked myself, how much do I remember? Though it seemed like a lot, the group discussion made me realise that it was hardly anything; most of what I remembered was either jumbled up with something else or was too vague and flimsy, and there was also quiet a lot of stuff that came out of no where...spontaneous generation? ha!...figments of my imagination!
We spoke about each of our perceptions on biology, a system, cells, DNA, genes and proteins, genetic splicing and what genetic engineering is all about. The theories on the origin of life and from where or how living cells came into existence, especially with so much diversity was another thing we discussed. Every theory makes sense in its own way, even with Pasteur's simple experiment to disprove the theory of spontaneous generation, no one can explain how the first cells, if there is a first that is, came into existence. As Karthik pointed out our brains are practically hardwired with the theory of cause and reaction, it is only natural that we assume that everything has an origin, things can't just pop out of nowhere. I personally believe that we don't know enough to disregard or discard seemingly irrational theories. Anything is possible.
The bbc documentary on cells gave us substantial insight into the history of the discovery of the cell and about the invention of the microscope; about Antonie van Leeuwenhoek and the other pioneers who planted major landmarks in the world of science.
We were also constantly trying to make sense of how we can contribute our skills as artists and designers to this world. When we started to learn about what genetic engineers did and about gene splicing and gene sequencing, it made sense, it's all an art; a gene sequence is like a colour, mixing different sequences would produce different results, i.e. different colours, the way you compose them on a canvas is the art. Once you get a hang of how to make the different colours, you can compose it anyway you like.
11th May 2010
I got my kiddie microscope to class today, it isn't too bad, magnifies up to 600x. We placed a droplet of muddy water on the slide and we were able to see what looked like little translucent worms, but we weren't sure if it was just the dust and scratches on the lens or if it was in the water. Then we tried placing a thin piece of sliced leaf and saw the pores on its surface. We tried making a microscope using an old Logitech webcam, it was interesting to know that all we had to do after dismantling the webcam was invert the lens! This made me realise that there's a lot of old stuff in all our homes which we could play around with and create new things; recycle and rebuild for some other purposes.
Then we watched videos on the Mendelian theory, how he observed and theorised the process of genetic inheritance using the pea plants, we were given examples of how this works in human beings. We learned about genomes, chromosomes and genes, a chromosome is sort of like a complex pack of genes. A gene in itself contains strands of DNA, which in turn contain pairs of bases-ATs and CGs.
For me, the coolest part was that Mendel was a monk and Antonie van Leeuwenhoek was an apprentice to a cloth merchant; and both of them are now seen as the forefathers of today’s Science. Growing up in a time when the Arts and the Sciences were perceived as immiscible (for the most part), it was empowering and reassuring to learn that just about anyone could be the next person to go down in history for discovering or coming up with an idea that could prove to be invaluable to the world.
17th May 2010
Apart from watching more videos about genetics, our task today was to create an imaginary creature. It could be absolutely useless and whimsical. These kinds of assignments are my personal favourite!
I've thought about this many times (although not in this context).Ever since I was little I remember imagining creatures; watching cartoons and TV shows with things that you wouldn't really see living on your planet makes you wonder and makes you dream about your own little (or really huge) creatures. Recently while watching Avatar I was constantly imagining things and I was really excited to do this exercise.
So my creature would be something that floats or flies. I wanted it to be translucent with a slight portion of its insides visible to us on the outside, I wanted it to be roughly the size of a medium sized bubble or a tennis ball. I wanted it to be a wobbly sphere.
Now, I didn't want my creature to be completely useless, since we are in great need of air purifiers, especially in big cities like Bangalore, why not create something that fed on the pollutants. Every time the pollution increased in the atmosphere these little creatures start sprouting out. It's their source of nutrition. I decided to name it Purgcaelum. It's latin for purify air (purg and caelum respectively).
I found that Nitrogen oxides are major pollutants, so I wanted Purgcaelum to be able to turn the nitrogen oxides into ammonia that could be helpful to plants. I wanted it to perform the role of nitrogen fixing bacteria! I'm not sure about how scientifically possible this task is though...
We're also doing a living art piece. Using any medium of portrayal, we're going to take a living thing and study it's pattern of growth or behaviour. As against cruelty towards living things as I am, I decided to study fireflies to see how I could control their behaviour (I will let them free ofcourse). Finding them around where I live however, is very difficult. I'm not even sure if it's their habitat.
After a lot of research, I found that sadly fireflies all around the world are endangered. Well, seeing a firefly in itself was a huge deal for me as a kid, forget catching one. I haven't seen one in years. It's extrememly sad. We should save them.
24th May 2010
Our previous ideas for creatures while crazy weren't crazy enough, so we set out to intensify their crazy quotient.
I thought up a whole new idea. And this is most definitely not possible, atleast won't be (I think) for many many years. And it is that, my creature is able to carry out the process of nuclear fission with in its body. It has a lining of concrete to protect its out membranes from exposure to the radiation and for it to coexist peacefully with the other organisms. Now why it needs this process is to create enough energy to produce enough pressure to survive both in shallow waters and in the deepest parts of the ocean. It has a highly developed pressure sensor.
Very little of the earth's seabed/ oceanbed has been explored by mankind due to the high amounts of pressure and this creature would be just the thing we need to look into the unexplored. It also has photogrpahic memory which we can easily access since its brain can be hacked into by humans. Again it is sphereical in shape with many pressure nodules on it's surface, it has eyes, a mouth and breathes as well. The nodules also act as the pressure sensors.
A colony of these creatures can be used to harvest energy to power whole cities.
Just got back to college today after four days of being ill, I missed out on the DNA extraction, but was explained the process of how it happens and how it looks. I never imagined it to be so simple, the very term 'DNA extraction' sounds like one needs an entire lab (like dexter's lab) to facilitate it!
This made me think on a more philosophical note; all the things that we think are really difficult, that are complicated and almost impossible to do are sometimes and most of the times the simplest and easiest to do, while the ones which we wrongly assume to be easy end up being really tough while doing them.
25th May 2010
We FINALLY went to the National Centre for Biological Sciences (NCBS) today! This place is bea-uti-full!!!!! It's so fancy, shiny labs, awesome rooftop cafes, and a dinning hall with inexpensive yummy food (or so I heard). We were supposed to meet up with Mukund, but couldn't since he was busy but we did get to meet another scientist, Shorab(I not sure about the spelling...) who spoke to us about the experiment he was working at that moment(literally, he'd even timed himself and the alarm went off for him to go back while he was in the middle of our discussion). It was to do with mice brains and their brain activity, he said a lot of things about the synapse, which I cannot recall since a very little of it made sense...but it did sound very interesting.
However what caught me was that he had to kill the mice before he worked on slices of their brain. He said that they're anesthetised before they're killed so I don't think they feel pain; they are not employing cruel methods, so that's good.
We met another scientist, who showed us around the labs and told us what the different machines do. It was amusing to find out that half the machines in the lab were only used to mix ingredients!
Also, we had a very interesting conversation in the morning at NCBS, it was about how a scientific institue could afford to be so luxurious while a majority of our country was starving and shelterless. How does our government prioritize? Does it even prioritize? We came up with many explainations to this, but I wonder, are we in a position, as a 3rd party, as outsiders to both the groups in question, to make assumptions about the situations. I think we need to listen to both stories and the government's story, and only then can we make a fair judgement.
30th May 2010
Finding fireflies is super hard! So while I'm still on the look out for them, I decided to try my music test on ants. I took two different kinds of ants. I'm not sure I can identify them because there are 632 different species of ants in India! All I can say is that on was a big black ant, with a longer body, while the other was smaller with a boxier body.
I put them in the glass bottle I'd saved for the fireflies with a two long leaves for company. The bigger one was more active on the whole, while the smaller one was mellow. I strated off playing really loud hip hop! It didn't seem to faze them much, the big one became more limited in it's exploration of the bottle while the smaller one hid under one of the leaves. It was very still through out. Next I gave them same quiet time. The big one resumed running around, the small one was still under the leaf. I started playing 3oh!3, then tweaked it by increasing the pitch (which was very very disturbing) and this time I played it on ear phones and I hung one ear phone into the bottle midway. Again they did the same thing, the little one was still under the leaf and the big one froze up on one of the leaves. I took the ear phone out and played it out loud, same reaction.
Now here's the interesting part, I played Explosions in the sky, who are a lot more soothing to human ears than 3oh!3 and the little guy actually came out from under the leaf, I don't know if it was just him getting used to the music but at one point it was as though he was coordinating his leg and antennae movement to the music. It probably was just coincidence or my imagination going wild but just to double check I'm going to repeat this experiment with different ants. Let's see what happens! However, I didn't see reaction as pronounced as I was hoping!
Moving on, hre's another idea. Instead of plastic, we could use bacteria. Bacteria which we could use in all the ways we use plastic, for bags, containers, clothes etc etc. The best part is that it's biodegradble! When the bacteria start dying we just put the product in a compost bin and use it as manure!
I had an inkling that there is already something like this out there, and yes there is, so take a look at this, it may not be exactly what I'm talking about but it's close enough to what I'm saying! http://www.scientificamerican.com/article.cfm?id=turning-bacteria-into-plastic-factories-replacing-fossil-fuels
1st June 2010
We tried to grow yeast today! We went up to the Aditi Lab and made some agar. We then tried colonising three different forms of yeast on the agar plates. One plate was smeared with dry yeast mixed with room temperature water, one with yeast in warm water and another with just dry yeast. We will leave it undisturbed for two days and see what happens!
2nd June 2010
Today, we went for a talk and presentation on Darwin's theory of evolution by Dr. Narsimhan at the National Institute of Advanced Studies. It was mainly a discussion about how much chance and how much design is involved in the making of the world. The presentation definitely brought on some questions and confusion, which I will write about in detail soon (which I will post in my Special entries section).
Later that day we went to NCBS again, we met Mukund (finally!) and he showed us around the area where we will be working. He also suggested that we make our own lab equipment! So this should be really cool! :)
3rd June 2010
We started work on building our lab equipment. We did some research on how to build incubators and centrifuges. I always felt that we needed something super fancy to make things happen but this course has kept surprising me with how much we can do with stuff we have lying around at home!
We had a look at what had happened to the yeast on the agar plates (to see if there were any colonies) and only one had some kind of growth, the one sprinkled with dry yeast was the only one that showed some kind of development while the rest were not so successful.
We also tried a new formula for the agar plates, we changed the agar:water proportions to see which one gave the best results. I think the one with 5g of agar to 250ml of water worked the best, it wasn't too dry and hard nor was it too gooey.
7th - 12th June 2010
This week was mostly about constructing our lab equipment, with the centrifuge not cooperating with our attempts at trying to make it work, it was a little crazy and at times frustrating. We got a table top mixer, we tried connecting a pipe to it's blade which we were hoping would spin, but the pipe was almost impossible to keep stable on the blade without making it permanent, and since we weren't sure about whether the rest of the idea would work or not, we needed something temporary, so after a lot of trial and error, we figured that all we need was the axis on which the blade rotated, if we could fix a round disc or metal trips on the part where the blade goes it would prove to be a lot easier, and as it happens it was a lot easier. We also had to deal with strong vibration and we were able to do away with them as well. Once the sealant used for covering the bottoms of the test tube holders has dried completely we will try this out, it should work.
Simultaneously, we made a hand held centrifuge using a hand blender. This was relatively easier to make since we had learned how to dodge certain problems from the table top version. It's quiet effective on chalk powder and poster paint but we still haven't tried it on actual DNA. I hope it works, this one's sort of like my baby :D.
The incubator was stubborn too, we only got it to maintain a near steady 37 degrees C on saturday. We realised that the thermocole ice box didn't really need aluminium foil lining and a light bulb beacuse these made the temperature rise over 100 C, with the clinical thermometer bursting! We also figured that it needed an industrial thermometer and four fans instead of the initially estimated, one. With four fans and a mosquito repellor providing heat our team mates were able to notice an almost steady temp. of 37 degrees C which was exactly around what we were hoping to achieve. Now all we need to do is see if the mosquito repellor affects the specimens in the petri dishes, if it does, then we will have to find an alternate source of low heat. I was thinking maybe a few Christmas lights or the little blubs used in torches, I think they would provide enough heat and we we could just use batteries to power it; making it more portable.
Can't wait for Monday!:)
14th, 15th and 16th June 2010
Alexandra and James are here! We presented a slideshow of our progress until now and they showed us their work.
On the first day, we spoke about our ideas and categorised them under the categories made by the Design for the First World competition. From what I gathered from the discussions that we had, trying to "solve" a problem is such a western thought and while critiquing western design it wouldn't really help us to think in exactly the same way that has been there. And this does make sense.
On the second day, we were asked to look up a previous Igem teams project and imagine that this idea was actually being put into effect sometime in the future. We chose the Team KULueven