Difference between revisions of "Preparing the pBAD and Lysis plasmids for gel electrophoresis"

From Hackteria Wiki
Jump to: navigation, search
Line 1: Line 1:
#Take 1.5ml of the culture in an epenndorff tube.
+
#Take 1.5µl of the culture in an epenndorff tube.
 
# Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
 
# Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
 
#The liquid around the collected pellet, called the supernatant can be discarded.
 
#The liquid around the collected pellet, called the supernatant can be discarded.
 
#Dissolve the pellet in 150µl of [[ALS I]]
 
#Dissolve the pellet in 150µl of [[ALS I]]
 
...
 
...

Revision as of 05:21, 9 June 2009

  1. Take 1.5µl of the culture in an epenndorff tube.
  2. Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
  3. The liquid around the collected pellet, called the supernatant can be discarded.
  4. Dissolve the pellet in 150µl of ALS I

...