Difference between revisions of "Preparing the pBAD and Lysis plasmids for gel electrophoresis"
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− | #Take 1. | + | #Take 1.5µl of the culture in an epenndorff tube. |
# Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube. | # Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube. | ||
#The liquid around the collected pellet, called the supernatant can be discarded. | #The liquid around the collected pellet, called the supernatant can be discarded. | ||
#Dissolve the pellet in 150µl of [[ALS I]] | #Dissolve the pellet in 150µl of [[ALS I]] | ||
... | ... |
Revision as of 05:21, 9 June 2009
- Take 1.5µl of the culture in an epenndorff tube.
- Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
- The liquid around the collected pellet, called the supernatant can be discarded.
- Dissolve the pellet in 150µl of ALS I
...