Difference between revisions of "Preparing the pBAD and Lysis plasmids for gel electrophoresis"

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(Created page with '#Take 1.5ml of the culture in an epenndorff tube. # Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in th...')
 
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# Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
 
# Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
 
#The liquid around the collected pellet, called the supernatant can be discarded.
 
#The liquid around the collected pellet, called the supernatant can be discarded.
#Dissolve the pellet in 150µl ALS I
+
#Dissolve the pellet in 150µl of[ALS I]
 +
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Revision as of 05:20, 9 June 2009

  1. Take 1.5ml of the culture in an epenndorff tube.
  2. Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
  3. The liquid around the collected pellet, called the supernatant can be discarded.
  4. Dissolve the pellet in 150µl of[ALS I]

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