Difference between revisions of "PCR your DNA Workshop"
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==Workflow== | ==Workflow== | ||
With a group of 15-20 people with everyone participating, we see that this workflow can easily take the entire day.<br> | With a group of 15-20 people with everyone participating, we see that this workflow can easily take the entire day.<br> | ||
− | + | You can stop and continue another time - after the DNA extraction and after the PCR<br> | |
# Get Samples | # Get Samples | ||
# Extract the DNA (PAUSE) | # Extract the DNA (PAUSE) |
Revision as of 13:52, 14 February 2016
How do we go from showing the existence of DNA (strawberries, soap and alcohol) to the popular imagery of DNA gels?
This is a consolidated practical go-to version of workshops we have done as Hack-a-Taq and Citizen Science @ Bern. It highlights also the generic lab equipment. You can find similar protocols in other places. This is our documentation.
Workflow
With a group of 15-20 people with everyone participating, we see that this workflow can easily take the entire day.
You can stop and continue another time - after the DNA extraction and after the PCR
- Get Samples
- Extract the DNA (PAUSE)
- Amplify the gene of your interest using PCR (PAUSE)
- Separate the PCR products by agarose gel electrophoresis
- Visualize the PCR products (your amplified DNA bits)