Difference between revisions of "Our Step-wise Process"

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(Created page with ''''Step 1 - Taking dry DNA from wells''' '''Step 2 - Transforming competent cells.''' ''Competent cells are those cells which have the ability to take up extracellular/n...')
 
Line 4: Line 4:
 
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.''
 
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.''
  
'''Step 3 - Pick a single colony.'''
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'''Step 3 - Picking a single colony.'''
  
'''Step 4 - Inoculate broth with Ampicillin-R''' (an antibiotic) and let it grow for 18 hours.
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'''Step 4 - Inoculainge broth with Ampicillin-R''' (an antibiotic) and letting it grow for 18 hours.
  
'''Step 5 - Use the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
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'''Step 5 - Using the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
  
 
'''Step 6 - [[Digesting the DNA]]'''
 
'''Step 6 - [[Digesting the DNA]]'''

Revision as of 16:04, 16 June 2009

Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.

Step 3 - Picking a single colony.

Step 4 - Inoculainge broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA