Difference between revisions of "Our Step-wise Process"
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(Created page with ''''Step 1 - Taking dry DNA from wells''' '''Step 2 - Transforming competent cells.''' ''Competent cells are those cells which have the ability to take up extracellular/n...') |
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''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ||
− | '''Step 3 - | + | '''Step 3 - Picking a single colony.''' |
− | '''Step 4 - | + | '''Step 4 - Inoculainge broth with Ampicillin-R''' (an antibiotic) and letting it grow for 18 hours. |
− | '''Step 5 - | + | '''Step 5 - Using the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' |
'''Step 6 - [[Digesting the DNA]]''' | '''Step 6 - [[Digesting the DNA]]''' |
Revision as of 16:04, 16 June 2009
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Picking a single colony.
Step 4 - Inoculainge broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA