Difference between revisions of "Our Step-wise Process"

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'''Step 6 - [[Digesting the DNA]]'''
 
'''Step 6 - [[Digesting the DNA]]'''
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'''Step 7 - [[Gel Electrophoresis]]'''

Latest revision as of 18:29, 16 June 2009

Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.

Step 3 - Picking a single colony.

Step 4 - Inoculainge broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA

Step 7 - Gel Electrophoresis