Difference between revisions of "Mukund's Brief Overview on Synthetic Biology Basics"

From Hackteria Wiki
Jump to: navigation, search
Line 12: Line 12:
  
 
[[File:DSC01403.JPG|200px|thumb|right|The discussion with Mukund]]
 
[[File:DSC01403.JPG|200px|thumb|right|The discussion with Mukund]]
 +
[[File:DSC01405.JPG|200px|thumb|right|Scattering of light (sketch)]]
 +
[[File:DSC01406.JPG|200px|thumb|right|Flourescence of light (sketch)]]
 +
  
 
There are three main ways by which light passes through an object:
 
There are three main ways by which light passes through an object:
  
 
a) Scattering  
 
a) Scattering  
[[File:DSC01405.JPG|200px|thumb|right|Scattering of light (sketch)]]
 
  
 
b) Absorption
 
b) Absorption
  
 
c) Flouresence
 
c) Flouresence
[[File:DSC01406.JPG|200px|thumb|right|Flourescence of light (sketch)]]
 
  
 
*There is one major problem with DNA, that is, its too small to see - even with high levels of  of magnification.Some of the ways of attempting to see them are through:
 
*There is one major problem with DNA, that is, its too small to see - even with high levels of  of magnification.Some of the ways of attempting to see them are through:
Line 27: Line 28:
 
b) Increasing the quantity of the sample observed
 
b) Increasing the quantity of the sample observed
  
Some basics of DNA Cloning:
+
'''Some basics of DNA Cloning:'''
 
+
'''Plasmid:'''
Plasmid:
 
  
 
A DNA molecule that is separate from the chromosomal DNA and is capable of replicating independently.
 
A DNA molecule that is separate from the chromosomal DNA and is capable of replicating independently.
  
Palindrome:
+
'''Palindrome:'''
  
 
A sequence that reads the same forward and backward.
 
A sequence that reads the same forward and backward.

Revision as of 05:37, 21 May 2009

Q- What will you see if you add mud to water?

A- Sedimentation and floating particles of mud; Darkish-colored water

Q-What will you see if DNA if added to water?

A-Clear water.

How does the phenomenon of 'seeing' the particles of mud and not the DNA take place? This is because the particles of mud scatter light that results in the eye being able to view them. DNA, however,allows light to pass right through it, due to which it becomes invisible to the eye.

The discussion with Mukund
Scattering of light (sketch)
Flourescence of light (sketch)


There are three main ways by which light passes through an object:

a) Scattering

b) Absorption

c) Flouresence

  • There is one major problem with DNA, that is, its too small to see - even with high levels of of magnification.Some of the ways of attempting to see them are through:

a) Addition of dyes b) Increasing the quantity of the sample observed

Some basics of DNA Cloning: Plasmid:

A DNA molecule that is separate from the chromosomal DNA and is capable of replicating independently.

Palindrome:

A sequence that reads the same forward and backward.

ATGCAT TACGTA

It is due to this characteristic of the DNA sequences that they can bind to other sequences.


Dna cloning.gif