Mobile Plant Analysis Lab @ pifCamp, Slovenia
This is part of the ongoing work during pifCamp on my Lab-in-the-Wild
DIY sap press
just use a garlic press :-)
What the heck is a "petiole"?
"In botany, the petiole is the stalk that attaches the leaf blade to the stem. The petiole is the transition between the stem and the leaf blade."
Most Common Methdos
Near Infrared Photography
as usual the people from publiclab have already done a great job!
A great use of our spectroscopes!
Atomic Absorbtion Spectroscopy
Quick Nitrate-N Tests
just published by pearce et al
but what about these enzymes? are they open source?
the classic method:
Measure Nitrate by reducing it to nitrite via Zinc in "alkalischem milieu".
i have the nitrite test...
Checking Trace Nitrate in Water and Soil Using an Amateur Scientist's Measurement Guide
Procedures and apparatus (photometer) for measuring concentrations of nitrates in water and soil using common equipment and materials; includes data and analysis.
Download here: File:amateurScience_n.pdf
Chlorophyl content and meters
Simple Cholorophyl extraction methods:
Hmmm somehow these different spectra found on the internet are a bit confusing. according to the standard chlorophyll is measured at 645 and 663 nm to quantify the chlorophyll a and b composition
Hmmmm, checking the MultispeQ again
Protein Content Colorimetric Assays
This link helped me a lot:
There are quite a lot out there. The Biuret test, testing peptide bonds with some copper-ions, and it's modifications the BCA and Lowry tests. most suitable for our first experiments seems to be the Bradford assay with it's color change from brown to blue.
- Bradford Reagent also known as Coomassie G-250 dye. in methanol
- still need a standard sample. but i got some BSA somewhere....
- Orange LEDs
- turbidity kits
Literature and Links
Handbook of Methods for Plant Analysis
Plant analyses are usually carried out on prepared samples in a laboratory under controlled conditions. Testing of forage samples may be conducted with near infrared spectrometry (NIR) in mobile laboratories. Plant tissue tests or quick tests can be conducted in the field on living plant tissue, plant sap, or fluid.
Not so sure about that...
Near-Infrared Spectroscopy in Food Analysis - Encyclopedia of Analytical Chemistry
Download here: File:NIR_food_analysis.pdf
Soil And Plant Analysis - Use A Lab Or Do It Yourself
SCiO: Explore More! A Pocket Molecular Sensor For All!