Difference between revisions of "Ligation"

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(Created page with '''' What is Ligation?''' Ligation is a process by which two DNA molecules are joined together in the pressence of a catalyst - DNA ligase, an ensyme.')
 
 
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Ligation is a process by which two DNA molecules are joined together in the pressence of a catalyst - DNA ligase, an ensyme.
 
Ligation is a process by which two DNA molecules are joined together in the pressence of a catalyst - DNA ligase, an ensyme.
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'''For June 15th:'''
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These bands will be kept overnight in 37 degrees Celsius.
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Then these will be run through a preparative gel which takes about 3 hours.
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The next step is to [[elute  the released products]] which wold take 1.5 hours.
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CIP treatment will be done
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[Calf Intestine Phosphate]
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This treatment is given to only the vector of Lysis (Process will take 1.5 hour)
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This is done so that the vector doesn't self ligate.
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This enzyme will then be inactivated at 65 degree C.
 +
 +
This vector needs to be purified using column or chloroform

Latest revision as of 05:57, 26 June 2009

What is Ligation?

Ligation is a process by which two DNA molecules are joined together in the pressence of a catalyst - DNA ligase, an ensyme.

For June 15th:

These bands will be kept overnight in 37 degrees Celsius.

Then these will be run through a preparative gel which takes about 3 hours.

The next step is to elute the released products which wold take 1.5 hours.

CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.

This enzyme will then be inactivated at 65 degree C.

This vector needs to be purified using column or chloroform