Difference between revisions of "Hack a Taq"
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[[File:Hack-a-taq.jpg|none|400px|]]<br><br> | [[File:Hack-a-taq.jpg|none|400px|]]<br><br> | ||
Now that there are several DIY PCR machines available, the next challenge was to find a reasonable source for Taq polymerase. Also, as a side-project for the [http://www.biodesign.cc BIO-DESIGN for the REAL WORLD] at EPFL, we wanted to see if we can make the "wet" part of DIY PCR more accessible, so that PCR could be used as a coliform bacteria detection method from water samples. <br> | Now that there are several DIY PCR machines available, the next challenge was to find a reasonable source for Taq polymerase. Also, as a side-project for the [http://www.biodesign.cc BIO-DESIGN for the REAL WORLD] at EPFL, we wanted to see if we can make the "wet" part of DIY PCR more accessible, so that PCR could be used as a coliform bacteria detection method from water samples. <br> | ||
+ | '''PLEASE NOTE''' that to use genetically modified bacteria, you will need to check and comply with your local/national regulations<br> | ||
This is a happy confluence of several items on the to-do list, and the process will be documented on [http://biodesign.cc biodesign.cc]<br><br><br> | This is a happy confluence of several items on the to-do list, and the process will be documented on [http://biodesign.cc biodesign.cc]<br><br><br> | ||
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* Taq protein extraction | * Taq protein extraction | ||
+ | |||
+ | == You will need == | ||
+ | == Equipment == | ||
+ | - heatable water bath | ||
+ | - water boiler/pot | ||
+ | - thermometer | ||
+ | - vortex | ||
+ | - magnetic stir plate | ||
+ | - magnet | ||
+ | |||
+ | - PCR conventional machine | ||
+ | - Wild OpenPCR machine | ||
+ | |||
+ | - pipettes | ||
+ | - shaker at 37C | ||
+ | - bunsen burner/ spirit lamp | ||
+ | |||
+ | - pressure cooker (for sterilization) | ||
+ | - heating | ||
+ | |||
+ | - agarose gel box | ||
+ | - weighing scale | ||
+ | - power source | ||
+ | - camera | ||
Revision as of 20:45, 20 March 2013
Now that there are several DIY PCR machines available, the next challenge was to find a reasonable source for Taq polymerase. Also, as a side-project for the BIO-DESIGN for the REAL WORLD at EPFL, we wanted to see if we can make the "wet" part of DIY PCR more accessible, so that PCR could be used as a coliform bacteria detection method from water samples.
PLEASE NOTE that to use genetically modified bacteria, you will need to check and comply with your local/national regulations
This is a happy confluence of several items on the to-do list, and the process will be documented on biodesign.cc
EDITING IN PROGRESS!!
Objectives
1. test the Wild OpenPCR machine (gaudilabs)
2. hack the Taq isolation protocol from Open Biotechnology, and see if we can make and purify the enzyme with alternative reagents from the consumable reagents sold there (hackteria open source bioart...)
3. test out some PCR primers to detect E. coli (biodesign for the real world)
Techniques Learned
- PCR
- agarose gel analysis of PCR products
- DNA extraction from E. coli
- Taq protein extraction
You will need
Equipment
- heatable water bath - water boiler/pot - thermometer - vortex - magnetic stir plate - magnet
- PCR conventional machine - Wild OpenPCR machine
- pipettes - shaker at 37C - bunsen burner/ spirit lamp
- pressure cooker (for sterilization) - heating
- agarose gel box - weighing scale - power source - camera
Daily Meeting Points
- 5 April - Optional pre-workshop day at EPFL
- 12:15pm (EPFL SV3.615) meet and work with the students of BIO-DESIGN for the REAL WORLD.
- 17:00 pm (EPFL) PUBLIC THESIS DEFENSE - Highthroughput 3D culture and analysis of stem cell differentiation (room and official title coming soon)
- 6 April
- 10:00 am (place de la Ripponne, steps of palace de rumine) OUTFITTING THE LAB shopping in the Riponne flea market
- 7 April
- 12 April - Optional pre-workshop day at EPFL
- 12:15pm (EPFL SV3.615) meet the students of BIO-DESIGN for the REAL WORLD.
- 13 April
- 10:00 am (place de la Ripponne, steps of palace de rumine) OUTFITTING THE LAB shopping in the Riponne flea market