Difference between revisions of "GlobalLAMP-Paris"

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<bR>[[File:GLAMP_CleanRoom.jpg|200px|right]]
 
<bR>[[File:GLAMP_CleanRoom.jpg|200px|right]]
 
Saturday, June 11th 2022<br>
 
Saturday, June 11th 2022<br>
- Visiting the different labs <br>
+
Visiting the different labs <br>
- Bringing materials to the lab<br>
+
Bringing materials to the lab<br>
- Taking over the house<br>
+
Taking over the house<br>
- Welcome beers
+
Welcome beers
- Lot's of bicycle riding<br>
+
Lot's of bicycle riding<br>
 
<br>
 
<br>
 
[[File:GLAMP_Welcome.jpg|200px|right]]
 
[[File:GLAMP_Welcome.jpg|200px|right]]
 
Sunday, June 12th<br>
 
Sunday, June 12th<br>
- chilling in the house<br>
+
chilling in the house<br>
- defining program for the week<br>
+
defining program for the week<br>
- tourist tour<br>
+
tourist tour<br>
- prepare kick-off presentation<br>
+
prepare kick-off presentation<br>
- dinner<br>
+
dinner<br>
 
<br>
 
<br>
Monday, June 13th<br>
+
 
- 9am: Kick-Off presentation<br>
+
[[File:GLAMP_Meeting.jpg|200px|right]]
- setting up the lab<br>
+
 
- starting lab work<br>
+
Monday, June 13th – Sharing Knowledge<br>
 +
Kick-Off presentation
 +
Setting up the lab<br>
 +
● Sensor Lab<br>
 +
● Lyophilization Intro<br>
 +
● Show and tell with prototypes<br>
 +
<br>
 +
[[File:GLAMP_Labwork.jpg|200px|right]]
 +
Tuesday, June 14th – Work Sessions<br>
 +
● Lab work session<br>
 +
● Hands On Workshops<br>
 +
● Amplification benchmark<br>
 +
<br>
 +
[[File:GLAMP_Network.jpg|200px|right]]
 +
Wednesday, June 15th – Networking<br>
 +
● Visiting local Hackerspaces / Fablabs<br>
 +
● Broadcasting from the lab<br>
 +
● Connecting with other GOSH communities<br>
 +
● Exchange with potential production partners<br>
 +
<br>
 +
[[File:GLAMP_Design.jpg|200px|right]]
 +
Thursday, June 16th – Design Sprints<br>
 +
● Rapid Prototyping<br>
 +
● Design <br>
 +
● Visualizations<br>
 +
<br>
 +
Friday, June 17th – Future Outlook<br>
 +
● Assessment of achievements<br>
 +
● Minimal viable products<br>
 +
● Global potential analysis <br>
 +
● Potential collaboration partners<br>
 +
● Planing for phase 2<br>
 +
● Open Houses Vincennes<br>
 +
<br>
 +
Saturday, June 18th – Guests & Celebrities<br>
 
<br>
 
<br>
 
 
More to come:<br>
 
More to come:<br>
 
- Test sensors
 
- Test the cameras
 
- Introduction in the process of Lyophilization
 
- Experiment with OpenDrop
 
- qPCR benchmark
 
-
 
 
 
 
- Food & Drinks? Cooking together?<bR>
 
    <bR>
 
 
Social Events<br>
 
Social Events<br>
- Social events1-2 day of visiting to other local communities<bR>
 
- Visiting to other local communities<bR>
 
- Visit a Hackerspace / FabLab / OpenLab in Paris<bR>
 
- Social evening with music and beers<bR>
 
<br>
 
  
 
One day of lectures<br>
 
One day of lectures<br>
Line 69: Line 85:
 
Seeed and some of the production companies?<br>
 
Seeed and some of the production companies?<br>
 
<br>
 
<br>
Workshops<br>
 
- Show and tell (bring your prototypes)<br>
 
- Technologies hand’s on (qPCR, LAMP, fluorescence detection, synthesisers<br>
 
 
- Future outlook session (where do we want to go with our project / applications / specifications)<br>
 
- Future outlook session (where do we want to go with our project / applications / specifications)<br>
 
- Field trip (going out to nature) to test devices / protocols in the field<br>
 
- Field trip (going out to nature) to test devices / protocols in the field<br>
  
Presentation:
+
Prototypes:
Ariel 9am - 10pm
 
 
 
- Who is here
 
- Showing some previous work and prototypes
 
 
   - Induction heating
 
   - Induction heating
 
   - PocketPCR / Pocket qPCR
 
   - PocketPCR / Pocket qPCR
Line 85: Line 94:
 
   - Webcam PCR
 
   - Webcam PCR
 
   - GMO/Corona Detective
 
   - GMO/Corona Detective
   - ChilloutLAMP
+
   - Chili LAMP
  -
 
  
 
- Program for the week
 
- Program for the week
Line 93: Line 101:
  
 
=== Locations ===  
 
=== Locations ===  
'''The Lab:'''<br>
+
'''The Lab:'''<br><br>
[[File:CRILab.jpg|300px]]<br>
+
[[File:lab.jpg|500px]]<br>
CRI (Center for Research and Interdisciplinarity)<br>
+
<br>
CRI is co-constructing and sharing new ways of learning, teaching, conducting research and mobilizing collective intelligence in the fields of life, learning and digital sciences, in order to address the UN’s sustainable development goals (SDGs).<br>
+
LPI (Learning Planet Institute) formerly CRI (Center for Research and Interdisciplinarity) <br>
 +
LPI is co-constructing and sharing new ways of learning, teaching, conducting research and mobilizing collective intelligence in the fields of life, learning and digital sciences, in order to address the UN’s sustainable development goals (SDGs).<br>
 
Address: Learning Planet Institute, 8bis rue Charles V, 75004, Paris.<br>
 
Address: Learning Planet Institute, 8bis rue Charles V, 75004, Paris.<br>
 
<br>
 
<br>
Line 106: Line 115:
 
49 Rue de la Liberté, Vincennes, Île-de-France 94300, France<br>
 
49 Rue de la Liberté, Vincennes, Île-de-France 94300, France<br>
 
<br>
 
<br>
 +
 +
=== Results ===
 +
 +
''' Devices designs '''
 +
<br>Chili qLAMP -> [https://gitlab.orosticaingenieria.cl/biopuc-group/lamp Gitlab] <br>
 +
WebCam Prototype -> [https://www.hackteria.org/wiki/index.php?title=QCamProto QCamProto] <br>
 +
Open qLAMP -> [https://gitlab.com/open-bioeconomy-lab/diagnostics-hardware/rt-lamp-device/-/tree/master/open_qLAMP Gitlab]
 +
 +
''' Prototypes benchmark '''
 +
 +
At the end of the workshop we compared the ability of the three prototypes (webcam qLAMP, chili qLAMP and [https://gitlab.com/open-bioeconomy-lab/diagnostics-hardware/rt-lamp-device/-/tree/master/open_qLAMP open qLAMP]) to amplify 200 SARS-CoV-2 inactivated viral units using the [https://pubmed.ncbi.nlm.nih.gov/35027866/ CoronaDetective] protocol in the green channel and the [GMO detective https://gmodetective.com/] in the red channel. The GMO should generate less signal as the filters we're using are for green fluorescence, which filters the vast majority of the red signal.
 +
 +
{| class="wikitable" border="1"
 +
|+ Prototypes benchmark results
 +
|-
 +
! WebCam qLAMP !! Chili qLAMP !! Open qLAMP
 +
|-
 +
|[[File:WebCam qLAMP POSH results.jpg|400px]]
 +
|[[File:chili qLAMP POSH results.jpg|600px]]
 +
|[[File:open qLAMP POSH results.jpg|400px]]
 +
|}
 +
 +
''' Pros and cons of each equipment '''
 +
 +
<u>Chili qLAMP</u>
 +
<br>
 +
From our experience Chili qLAMP is a really sensible piece of hardware. It really reach levels of sensitivity that the other two machines struggle to sense, but it still require some calibration to find a compromise between detecting low levels of product fluorescence without getting saturated by the negative controls.
 +
 +
With a price of 20€ per each photodiode, Boris will work in the short term to find a greater price without sacrificing the high sensitivity.
 +
 +
Also, the inclusion of a Raspberry Pi inside of the machine allows to do a high level processing of results and connect with other devices in the network.
 +
<br>
 +
<br>
 +
<u>WebCam qLAMP</u>
 +
<br>
 +
Eventhought it's still early in its development, the webcam qlamp have demosntrated the ability to generate really good results just using a 2/3$ camera as the sensor unit.
 +
 +
This machine is really promising for future applications as measuring multiplexed fluorescences in one single tube (Different wavelengths for measuring different things at the same time in one reaction), or scaling the format to 96/384 plates without an additional cost.
 +
<br>
 +
<br>
 +
<u>open qLAMP</u>
 +
<br>
 +
The open qLAMP is a middle point between the two previous machines. It uses an photodiode analog circuit but by the price range of the webcam qlamp.
 +
 +
It's the most ready for production device at the time of this residence. It still require some adjustments as including an small screen for controlling the machine when a local network is not available and developing an standard and quicker calibration procedure.
 +
<br>
 +
[[File:Assembled.jpg|800px]]
 +
<br>
 +
''' Future perspectives '''
 +
 +
Last days' topic consisted in how to move forward into real world applications. The following questions arose:
 +
 +
- '''The system comprises two main parts''', the [https://www.protocols.io/view/freeze-drying-lyophilization-and-manufacturing-of-5jyl85rq7l2w/v2 lyophilized reactions] and the hardware. Yanwu has contacts in the Chinese production ecosystem and will explore how to scale the reactions production. For the Hardware, Boris, Urs and Fran will study how to build the devices on a mid to large-scale and sell them. <br>
 +
 +
- '''Regarding the application, we discussed RED (Research, Education and Diagnosys) applications'''. Initially, Research and Education would be more straightforward targets. Still, we believe that open source affordable devices can impact decentralized diagnostics. For Education, we can always use the GMO detective. <br>
 +
 +
- '''For choosing a new target''' (as the pandemic is apparently ending), Harry will study the needs in Ghana. We talked about typhoid fever. As pointed [https://link.springer.com/content/pdf/10.1007/s41669-019-0159-7.pdf here], there is a need for a cheap and specific diagnosis. The current Widal test is unspecific and can generate many false positives, leading to unnecessary treatments and increased antibiotic resistance to the standard treatment. A test that costs less than 4$ per person is needed. DNA detective reactions cost 0.5€ to be produced.<br>
 +
 +
- '''Plant or animal diagnosis''' are easier to have a certified than human diseases diagnosys. Funghi detective, developed at CRI (For the pathogens generating the [https://www.cabi.org/ISC/abstract/19980614436 Ink Disease] in trees), can be an example. Still, we will need feedback on which agricultural diseases - are essential to be diagnosed on time.<br>
 +
 +
- '''How we will organize it?''' For round 2, we will create an association where we can transparently receive the funds, and everyone has control over them. For the future, we think the better way is a local company network where different participants create different open-source products required by others in the network. In this way, for example, Urs, Fran or Boris will build the hardware, Yanwu will produce the reactions that will be sent to Duplex Bioscience, the local company Harry has created to distribute in Ghana the technology.<br>
 +
 +
<br>
 +
 +
{| class="wikitable" border="0"
 +
|-
 +
|[[File:meeting.jpg|600px]]
 +
|[[File:workshop.jpg|640px]]
 +
|}
 +
 
===  Event Funded by: ===  
 
===  Event Funded by: ===  
 
* GOSH Collaborative Development Program<br>
 
* GOSH Collaborative Development Program<br>

Latest revision as of 09:07, 29 June 2022

GlobalLAMPSticker.png

Collaborative Developement of Isothermal Amplification & Devices
June 11. - 19, 2022 Paris

Scope of the Project

Quantitative real-time DNA amplification is at the heart of either diagnosing infectious disease or any section of biology research. Use of these devices has been limited due its cost and availability, therefore, refuting to elaborate and end-point methods like gel-electrophoresis. This project will change accessibility and affordability of this device to specially low-income countries either for POC or teaching purposes. Furthermore, the core of the project includes researchers and collaborators from diverse geographical backgrounds, professional expertise and socioeconomic origins, either at the development stage or at the end-user level at the next phases.

[Project Application [1]]

Participants
- Francisco Javier Quero (France)
- Urs Gaudenz (Switzerland)
- Yanwu Guo (Oslo)
- Fernán Federici Noe (Chile)
- Boris Enrique Orstica Navarrete (Chile)
- Harry Akligoh (Ghana)
- Ganga Chinna Rao Devarapu (remote)
- Smitha S Hegde (remote)
- Marc Dusseiller (remote / Indonesia)
- Shingo Hisakawa (remote Japan)

Programm


GLAMP CleanRoom.jpg

Saturday, June 11th 2022
● Visiting the different labs
● Bringing materials to the lab
● Taking over the house
● Welcome beers ● Lot's of bicycle riding

GLAMP Welcome.jpg

Sunday, June 12th
● chilling in the house
● defining program for the week
● tourist tour
● prepare kick-off presentation
● dinner

GLAMP Meeting.jpg

Monday, June 13th – Sharing Knowledge
● Kick-Off presentation Setting up the lab
● Sensor Lab
● Lyophilization Intro
● Show and tell with prototypes

GLAMP Labwork.jpg

Tuesday, June 14th – Work Sessions
● Lab work session
● Hands On Workshops
● Amplification benchmark

GLAMP Network.jpg

Wednesday, June 15th – Networking
● Visiting local Hackerspaces / Fablabs
● Broadcasting from the lab
● Connecting with other GOSH communities
● Exchange with potential production partners

GLAMP Design.jpg

Thursday, June 16th – Design Sprints
● Rapid Prototyping
● Design
● Visualizations

Friday, June 17th – Future Outlook
● Assessment of achievements
● Minimal viable products
● Global potential analysis
● Potential collaboration partners
● Planing for phase 2
● Open Houses Vincennes

Saturday, June 18th – Guests & Celebrities

More to come:
Social Events

One day of lectures
Invite and connect to other GOSH communities
Public / broadcasted event / time for broadcast in the lab
Seeed and some of the production companies?

- Future outlook session (where do we want to go with our project / applications / specifications)
- Field trip (going out to nature) to test devices / protocols in the field

Prototypes:

 - Induction heating
 - PocketPCR / Pocket qPCR
 - open qLAMP
 - Webcam PCR
 - GMO/Corona Detective
 - Chili LAMP

- Program for the week - How to collaborate in the future - Say hello with Marc (Indonesia) and Shingo (Japan)

Locations

The Lab:

Lab.jpg

LPI (Learning Planet Institute) formerly CRI (Center for Research and Interdisciplinarity)
LPI is co-constructing and sharing new ways of learning, teaching, conducting research and mobilizing collective intelligence in the fields of life, learning and digital sciences, in order to address the UN’s sustainable development goals (SDGs).
Address: Learning Planet Institute, 8bis rue Charles V, 75004, Paris.

Our Home-Stay:
Screenshot from 2022-06-09 12-05-16.png
House in the city with backyard, hosted by Alexandra
https://www.google.com/maps?daddr=48.849639892578125,2.443648099899292
49 Rue de la Liberté, Vincennes, Île-de-France 94300, France

Results

Devices designs
Chili qLAMP -> Gitlab
WebCam Prototype -> QCamProto
Open qLAMP -> Gitlab

Prototypes benchmark

At the end of the workshop we compared the ability of the three prototypes (webcam qLAMP, chili qLAMP and open qLAMP) to amplify 200 SARS-CoV-2 inactivated viral units using the CoronaDetective protocol in the green channel and the [GMO detective https://gmodetective.com/] in the red channel. The GMO should generate less signal as the filters we're using are for green fluorescence, which filters the vast majority of the red signal.

Prototypes benchmark results
WebCam qLAMP Chili qLAMP Open qLAMP
WebCam qLAMP POSH results.jpg Chili qLAMP POSH results.jpg Open qLAMP POSH results.jpg

Pros and cons of each equipment

Chili qLAMP
From our experience Chili qLAMP is a really sensible piece of hardware. It really reach levels of sensitivity that the other two machines struggle to sense, but it still require some calibration to find a compromise between detecting low levels of product fluorescence without getting saturated by the negative controls.

With a price of 20€ per each photodiode, Boris will work in the short term to find a greater price without sacrificing the high sensitivity.

Also, the inclusion of a Raspberry Pi inside of the machine allows to do a high level processing of results and connect with other devices in the network.

WebCam qLAMP
Eventhought it's still early in its development, the webcam qlamp have demosntrated the ability to generate really good results just using a 2/3$ camera as the sensor unit.

This machine is really promising for future applications as measuring multiplexed fluorescences in one single tube (Different wavelengths for measuring different things at the same time in one reaction), or scaling the format to 96/384 plates without an additional cost.

open qLAMP
The open qLAMP is a middle point between the two previous machines. It uses an photodiode analog circuit but by the price range of the webcam qlamp.

It's the most ready for production device at the time of this residence. It still require some adjustments as including an small screen for controlling the machine when a local network is not available and developing an standard and quicker calibration procedure.
Assembled.jpg
Future perspectives

Last days' topic consisted in how to move forward into real world applications. The following questions arose:

- The system comprises two main parts, the lyophilized reactions and the hardware. Yanwu has contacts in the Chinese production ecosystem and will explore how to scale the reactions production. For the Hardware, Boris, Urs and Fran will study how to build the devices on a mid to large-scale and sell them.

- Regarding the application, we discussed RED (Research, Education and Diagnosys) applications. Initially, Research and Education would be more straightforward targets. Still, we believe that open source affordable devices can impact decentralized diagnostics. For Education, we can always use the GMO detective.

- For choosing a new target (as the pandemic is apparently ending), Harry will study the needs in Ghana. We talked about typhoid fever. As pointed here, there is a need for a cheap and specific diagnosis. The current Widal test is unspecific and can generate many false positives, leading to unnecessary treatments and increased antibiotic resistance to the standard treatment. A test that costs less than 4$ per person is needed. DNA detective reactions cost 0.5€ to be produced.

- Plant or animal diagnosis are easier to have a certified than human diseases diagnosys. Funghi detective, developed at CRI (For the pathogens generating the Ink Disease in trees), can be an example. Still, we will need feedback on which agricultural diseases - are essential to be diagnosed on time.

- How we will organize it? For round 2, we will create an association where we can transparently receive the funds, and everyone has control over them. For the future, we think the better way is a local company network where different participants create different open-source products required by others in the network. In this way, for example, Urs, Fran or Boris will build the hardware, Yanwu will produce the reactions that will be sent to Duplex Bioscience, the local company Harry has created to distribute in Ghana the technology.


Meeting.jpg Workshop.jpg

Event Funded by:

  • GOSH Collaborative Development Program

https://forum.openhardware.science/t/apply-here-for-gosh-s-2022-collaborative-development-program-round-1/3380/15

  • Shuttleworth Foundation Flash Grants

https://shuttleworthfoundation.org/fellows/flash-grants/?fbclid=IwAR2o2UeCjrwHJErgZYmbreuHWnLbdm6qHgZNJwVD6kf04bLl1uoXy6bGg9E

  • Tsinghua University, Materials sponsor
  • CRI, Center for Research and Interdisciplinarity


Thank you to our sponsors