Difference between revisions of "DIY microscopy"

From Hackteria Wiki
Jump to: navigation, search
(Magnification and Field of View =)
Line 14: Line 14:
 
Movie recorded with modified webcams:
 
Movie recorded with modified webcams:
 
[http://www.youtube.com/watch?v=uIFMqmSjTMc&feature=channel_page Hackteria in Berlin]
 
[http://www.youtube.com/watch?v=uIFMqmSjTMc&feature=channel_page Hackteria in Berlin]
=== Magnification and Field of View ====
+
==== Instructions ====
 +
 
 +
 
 +
''Step1:''
  
See: [[fieldofview]]
 
  
==== Instructions ====
+
==== Magnification and Field of View ====
 +
 
 +
See: [[fieldofview]] for more details.
  
 +
after completing the hack with the lenses, the magnification can be increased upto a field of view of 0.7 mm.
  
''Step1:''
 
  
  
 
==== more about microscopy ====
 
==== more about microscopy ====

Revision as of 09:51, 6 June 2009

DIY microscopy

Introduction

To use a simple usb webcam as a microscope, only a few modifications are needed.

Diy microscope steps.jpg

In short, a small hack to the optics of a standard webcam with an adjustable focus-lens, allows to create video data, with a magnification of around 100 to 400x (depending on the model) at a working distance of a few mm. The highest magnifications can be achieved by inverting (putting upside down) the lens. Addition of good lighting by the use of leds allows to create images using a bright-field method (shine through the stuff) or dark field method (look at the reflections and scattering).

dark-field image sequence (4 sec) of an amoebae recorded on a modified webcam
bright-field image sequence (4 sec) of an amoebae recorded on a modified webcam

Movie recorded with modified webcams: Hackteria in Berlin

Instructions

Step1:


Magnification and Field of View

See: fieldofview for more details.

after completing the hack with the lenses, the magnification can be increased upto a field of view of 0.7 mm.


more about microscopy