Difference between revisions of "DIY Water Bath"

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'''PROCESS PICTURES:'''
 
'''PROCESS PICTURES:'''
  
[[File:DSC03963.JPG]]
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[[File:DSC03963.JPG|frame|none|Remove the glass casing of the aquarium heater]]
[[File:DSC03967.JPG‎]]
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[[File:DSC03967.JPG‎|frame|none|Unscrew the connecter screw]]
[[File:DSC03974.JPG‎]]
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[[File:DSC03974.JPG‎|frame|none|use it to pull out the thermostat adjustment]]
[[File:DSC03980.JPG‎]]
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[[File:DSC03980.JPG‎|frame|none|With sand paper file the tip of the thermostat adjustment. Be careful as every mm that you file off increases the temperature greatly]]
[[File:DSC03988.JPG]]
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[[File:DSC03988.JPG|frame|none|Once the temperature is achieved scew it the same way it was opened]]
[[File:DSC03990.JPG‎]]
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[[File:DSC03990.JPG‎|frame|none|place it into the glass casing and properly seal the edge]]
[[File:DSC03993.JPG‎]]
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[[File:DSC03993.JPG‎|frame|none|]]
[[File:DSC03997.JPG‎]]
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[[File:DSC03997.JPG‎|frame|none|clean the bucket with detol]]
[[File:DSC03998.JPG]]
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[[File:DSC03998.JPG|frame|none|]]
[[File:DSC04003.JPG‎]]
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[[File:DSC04003.JPG‎|frame|none|half fill with boiled water.]]
[[File:DSC04006.JPG]]
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[[File:DSC04006.JPG|frame|none|]]
[[File:DSC04013.JPG]]
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[[File:DSC04013.JPG|frame|none|Attach a lid to the bucket]]
[[File:DSC04015.JPG]]
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[[File:DSC04015.JPG|frame|none|]]
[[File:DSC04023.JPG]]
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[[File:DSC04023.JPG|frame|none|]]
[[File:DSC04028.JPG]]
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[[File:DSC04028.JPG|frame|none|Make a small hole on the lid for the thermometer]]
[[File:DSC04031.JPG]]
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[[File:DSC04031.JPG|frame|none|Stick the aquarium heater in the bucket and make shure that the heating element is completely submerged]]
[[File:DSC04032.JPG‎]]
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[[File:DSC04032.JPG‎|frame|none|]]
[[File:DSC04035.JPG]]
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[[File:DSC04035.JPG|frame|none|]]
[[File:DSC04040.JPG]]
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[[File:DSC04040.JPG|frame|none|And it's ready]]

Revision as of 07:30, 17 October 2010

A laboratory water bath is a tool used to maintain a very stable temperature much like an incubator. It is used to give indirect heat to the chemicals or bacteria and maintains that temperature. Water baths can hold often temperatures within a tenth of a degree Celsius; the water is often circulated. Sometimes beads are used as a waterless option. We use it to give bacteria heat shock thus loosening its cell wall. Our experiment needs a waterbath constant at 42°C so the maximum temperature of the waterbath.


You will need,


1. Bucket.........................DSC03955.JPG


2. Aquarium heater............DSC03953.JPG


3. Tape...........................DSC03956.JPG


4. Thermocole sheet...........DSC03960.JPG


5. Thermometer.................Thermometer.JPG


6. Boiled Water


How our water bath works

Our water bath uses a simple aquarium heater who's thermostat is hacked to cutoff at 42°C. A simple bucket is used as the container for the water bath as it does not allow the heat to escape.


PROCEDURE:

1. Remove the glass casing of the aquarium heater without breaking it.

2. Unscrew the connecter screw and use it to pull out the thermostat adjustment. Be careful not to break it, just bend it slightly.

3. With sand paper file the tip of the thermostat adjustment. Be careful as every mm that you file off increases the temperature greatly.

4. Keep filing and checking till the right temperature is achieved. (In our case it was 42 C. and was achieved after about 5 tries.)

5. Once the temperature is achieved seal off the heater the same way you opened it and place it into the glass casing and properly seal the edge so that there is no chance of water seeping in.

6. Next clean the bucket with detol and half fill with boiled water.

7. Attach a lid to the bucket to reduce heat loss.

8. Make a small hole on the lid for the thermometer. It is important that the thermometer is dipped in the water.

9. Stick the aquarium heater in the bucket and make shure that the heating element is completely submerged. For safety do not put the opened side underwater.

10. You can make the float using thermocol with holes bog enough for the vials to fit into. Your water bath is ready.


PRECAUTIONS:

1. Try not to crack the outer casing while opening it for the first time. I know it is difficult but try.

2. Never let the water touch the circuit or the heating element

3. Do not dip hot glass in water it will crack and the circuit will burst.

4. Do not touch heating coil after use.

5. Be careful to not file the thermostat adjustment too much or you will have to shut it down manually every time it goes above the required temperature.

6. Only half fill the bucket so that water doesn’t touch the open side of the circuit.


PROCESS PICTURES:

Remove the glass casing of the aquarium heater
Unscrew the connecter screw
use it to pull out the thermostat adjustment
With sand paper file the tip of the thermostat adjustment. Be careful as every mm that you file off increases the temperature greatly
Once the temperature is achieved scew it the same way it was opened
place it into the glass casing and properly seal the edge
DSC03993.JPG
clean the bucket with detol
DSC03998.JPG
half fill with boiled water.
DSC04006.JPG
Attach a lid to the bucket
DSC04015.JPG
DSC04023.JPG
Make a small hole on the lid for the thermometer
Stick the aquarium heater in the bucket and make shure that the heating element is completely submerged
DSC04032.JPG
DSC04035.JPG
And it's ready