Difference between revisions of "DIY Laser tweezer, cell trap, oligo synthesis"
(Created page with 'Started rebuilding the optical tweezers. First Notes: - The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather...') |
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| − | Started rebuilding the optical tweezers. | + | Started rebuilding the optical tweezers.<br> |
| − | + | <br> | |
| − | First Notes: | + | First Notes:<br> |
| − | - The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather. | + | - The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather.<br> |
| − | - The suggested current of 350mA for a DVD laser diode is at the upper limit of what most of these laser can take (without extra cooling). So go slowly and expect to blow up your diode at 350 mA (what happend to me) | + | - The suggested current of 350mA for a DVD laser diode is at the upper limit of what most of these laser can take (without extra cooling). So go slowly and expect to blow up your diode at 350 mA (what happend to me)<br> |
| − | - Generaly laser diodes are quite delicate. When they suddenly get less bright then they are in the so called "LED" mode an kaput. | + | - Generaly laser diodes are quite delicate. When they suddenly get less bright then they are in the so called "LED" mode an kaput.<br> |
| − | + | <br> | |
| − | + | <br> | |
| − | + | [[File:trap_schema.jpg|none|400px|]]<br> | |
| + | <br> | ||
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Revision as of 10:13, 31 August 2012
Started rebuilding the optical tweezers.
First Notes:
- The schematic in the papaer to power the laser uses an "unusual" pinout of the LM317. Refer to the schematic below rather.
- The suggested current of 350mA for a DVD laser diode is at the upper limit of what most of these laser can take (without extra cooling). So go slowly and expect to blow up your diode at 350 mA (what happend to me)
- Generaly laser diodes are quite delicate. When they suddenly get less bright then they are in the so called "LED" mode an kaput.
Single-cell isolation using a DVD optical pickup A. Kasukurti, M. Potcoava, S.A. Desai, C. Eggleton, and D. W. M. Marr1,* http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169297/
Optical tweezers directed one-bead one-sequence synthesis of oligonucleotides† http://www.ncbi.nlm.nih.gov/pubmed/21445444
Interferometry with optical pickups.
Quercioli F, Tiribilli B, Bartoli A.
http://www.ncbi.nlm.nih.gov/pubmed/18073818
A CHEAP 2D FLUORESCENCE DETECTION SYSTEM FOR μM-SIZED BEADS ON-CHIP Loes I. Segerink*, Maarten J. Koster, Ad J. Sprenkels, Istvan Vermes and Albert van den Berg
A new DNA chip detection mechanism using optical pick-up actuators Kyung-Ho Kim, Seung-Yop Lee, Sookyung Kim, Seung-Hwan Lee and Seong-Gab Jeong http://www.springerlink.com/content/084133p3n5mk1411/
