DIY $25 Gel Box
The Gel-Electrophoresis-Machine is constructed by creating a chamber that holds an electrolyte(salt water) that allows the conduction of electricity. A potential would be applied across it. Within, an agarose gel is placed and this also experiences the potential applied on the ends of the chamber. In the gel are wells containing substances of interest that have a know charge. Potential is applied in order to pull the substance through the agarose gel. It is apparent that the smaller molecules would travel further through the gel than the larger molecules. Thus, with a ladder-a set of molecules with known DNA lengths or molecule size, comparison would allow us to determine the length/size of the molecule of interest.
We constructed a functional Gel Electrophoresis Chamber in a span of a day using :
5.Black and red Test Lead Wire
7.Heat shrinking tubing, one black and one red
The Materials used:
The Building Process:
Gel Electrophoresis using Food Dyes: