Difference between revisions of "DIY $25 Gel Box"

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The Gel-Electrophoresis-Machine is constructed by creating a chamber that holds an electrolyte(salt water) that allows the conduction of electricity. A potential would be applied across it. Within, an agarose gel is placed and this also experiences the potential applied on the ends of the chamber.
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In the gel are wells containing substances of interest that have a know charge. Potential is applied in order to pull the substance through the agarose gel. It is apparent that the smaller molecules would travel further through the gel than the larger molecules. Thus, with a ladder-a set of molecules with known DNA lengths or molecule size, comparison would allow us to determine the length/size of the molecule of interest.
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We constructed a functional Gel Electrophoresis Chamber in a span of a day using :
 
We constructed a functional Gel Electrophoresis Chamber in a span of a day using :
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Materials:
 
Materials:
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'''The Materials used:'''
 
'''The Materials used:'''
 
<gallery>
 
<gallery>
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File:Materials.jpg |Different Dimensions of Plexiglass
 
File:Materials.jpg |Different Dimensions of Plexiglass
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'''The Building Process:'''
 
'''The Building Process:'''
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<gallery>
 
<gallery>

Revision as of 09:51, 1 June 2009

The Gel-Electrophoresis-Machine is constructed by creating a chamber that holds an electrolyte(salt water) that allows the conduction of electricity. A potential would be applied across it. Within, an agarose gel is placed and this also experiences the potential applied on the ends of the chamber. In the gel are wells containing substances of interest that have a know charge. Potential is applied in order to pull the substance through the agarose gel. It is apparent that the smaller molecules would travel further through the gel than the larger molecules. Thus, with a ladder-a set of molecules with known DNA lengths or molecule size, comparison would allow us to determine the length/size of the molecule of interest.

We constructed a functional Gel Electrophoresis Chamber in a span of a day using :


Materials:

1.Plexiglass

2.Teflon

3.Banana Jacks

4.Banana Plugs

5.Black and red Test Lead Wire

6.Copper wire

7.Heat shrinking tubing, one black and one red

The Materials used:

The Building Process:


June 1st

Gel Electrophoresis using Food Dyes: