Back to Bombyx

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Silk Worms are Back!
Bombyx mori is the domesticated silk worm bred for silk production.

Methods

Obtaining Silk Protein

  1. Degumming or removal of sericin - 5g/L sodium carbonate, pH, T, 1h (for Bombyx).
  2. Dissolution of fibroin

Step-by-step documentation from HTGAA 2015 from silklab

LiBr or not

Lithium Bromide (LiBr), while one can source it online, as a non-institutional person, still can be hard to find.
Brief summary of reading some papers is that LiBr is the best for degummed fibers of silk cocoons, where nearly 100% can be solubilized in LiBr, compared to 30% in CaCl2, or 50% NaSCN (sodium thiocyanate) (numbers taken from Addis and Raina 2013).
A Researchgate thread mentioned "2.5wt% silk fibroin in 28% ammonium aqueous solution by magnetic stirring."

Sourcing

CH

IN

  • Department of Sericulture Government of Karnataka
  • Silkworm Seed Production Centre, Government Office Bengaluru, Karnataka
  • National Silkworm Seed Organization, Agricultural Seed Store, Bengaluru, Karnataka
  • Silk Worm Breeding Unit, Bengaluru, Karnataka
  • Sri Lingeshwara Silk Worm Seed Product, Kamenahalli, Karnataka

References

About the Silk Worm

Material Properties and Methods

  • Polyol-Silk Bioink Formulations as Two-Part Room-Temperature Curable Materials for 3D Printing ACS Biomater. Sci. Eng., 2015, 1 (9), pp 780–788 DOI: 10.1021/acsbiomaterials.5b00160: The researchers combined silk proteins, which are biocompatible, and glycerol, a non-toxic sugar alcohol commonly found in food and pharmaceutical products. The resulting ink was clear, flexible, stable in water, and didn’t require any processing methods.
  • Pdf of Materials fabrication from Bombyx mori silk fibroin, Nat Protoc. 2011 Sep 22;6(10):1612-31. doi: 10.1038/nprot.2011.379.
  • HTGAA 2015 Lucern documentation on bioprinting

Molecular Biology