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Silk Worms are Back!

Methods

Obtaining Silk Protein

Step-by-step documentation from HTGAA 2015 from silklab

Sourcing

CH

IN

  • Silkworm Seed Production Centre, Government Office Bengaluru, Karnataka
  • National Silkworm Seed Organization, Agricultural Seed Store, Bengaluru, Karnataka
  • Silk Worm Breeding Unit, Bengaluru, Karnataka
  • Sri Lingeshwara Silk Worm Seed Product, Kamenahalli, Karnataka

References

Material Properties and Methods

  • Polyol-Silk Bioink Formulations as Two-Part Room-Temperature Curable Materials for 3D Printing ACS Biomater. Sci. Eng., 2015, 1 (9), pp 780–788 DOI: 10.1021/acsbiomaterials.5b00160: The researchers combined silk proteins, which are biocompatible, and glycerol, a non-toxic sugar alcohol commonly found in food and pharmaceutical products. The resulting ink was clear, flexible, stable in water, and didn’t require any processing methods.
  • [2014.igem.org/wiki/images/4/4e/Silk_Materials_Protocol_Paper.pdf Pdf] of Materials fabrication from Bombyx mori silk fibroin, Nat Protoc. 2011 Sep 22;6(10):1612-31. doi: 10.1038/nprot.2011.379.
  • HTGAA 2015 Lucern documentation on bioprinting

Molecular Biology

  • SilkDB a draft genome sequence for the silkworm B. mori, by the Beijing Genomics Institute (BGI)
  • SilkDB for the silkworm, Bombyx mori from Scripps and , genomic data including functional annotation of genes, gene products and chromosomal mapping, extensive biological information such as microarray expression data, ESTs and corresponding references
  • WildSilkbase BLAST searchable catalogue of expressed sequence tags (ESTs) generated from several tissues of wild silkmoths at different developmental stages, Center for DNA Fingerprinting and Diagnosis, Hydrabad India
  • Insect Proteome Database, Silkworm
  • Table 2.2 Genomics and Functional Genomics Resources Molecular Biology and Genetics of the Lepidoptera edited by Marian R. Goldsmith, Frantisek Marec, CRC Press ISBN 1420060201, 9781420060201.