ArtScience IGEM team
As amateurs/novices,we hope to bring our training in the arts and design as 'outsiders' and (hopefully) critical thinkers to synthetic biology, which, no doubt is a powerful tool and paradigm in looking at the life sciences. During the course of the workshop we hope to not only learn the tools and techniques of synthetic biology and come up with a piece of life which reflects our concerns but also use the process to engage with the political, ethical and cultural implications of Synthetic Biology.
-bacteria that prevents corrosion
-Time keeper or clock
-Related to weather - smell of rain
-Bacteria that is resistant to Scientific Probing/Instruments
-Mirror of bacteria
-Another creature made from bacteria
-Bacteria and sound
- Bacteria visualizations. (Both colour changes and 'choreographed' movement)
-Neuro transmitters- bacteria as sensors for emotions
-Bacteria that detects cravings
-Self mutating Bacteria
-Bacteria creates an identity
-Bacteria becoming material on death
-Constructing a 'Bacterial Ecology'
-Bacteria that acts like oil
- A lubricant - A liquid that can withstand high temperatures
- Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
- Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.
Images of the various results attained:
For June 14th:
- (Lysis= K112808)
pBAD- promoter Lysis-big construct- gene for cell lysis Gel image of released pBAD(I0500), after digestion with EcoRI+PstI:
Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.
pBAD is digested with EcoRI +SPeI Lysis is digested with EcoRI + XbaI
A small interlink sequence between E and X will break open and spread as one band
For June 15th:
These bands will be kept overnight in 37 degrees Celsius.
Then these will be run through a preparative gel which takes about 3 hours.
The next step is to elute the released products which wold take 1.5 hours.
CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.
This enzyme will then be inactivated at 65 degree C.
This vector needs to be purified using column or chloroform
Please look up this link before Monday. Special attention to restriction enzymes while you are at it! http://partsregistry.org/Assembly:Standard_assembly
It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.
The IBB Pune Team's wiki 
Art and Politics
Claire Pentecost::Beyond Face
Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt
Donna Haraway::Speculative Fabulations for Technoculture's Generations
Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life
GeneAeshetics, The Art of Joe Davis
Designer Bodies: Towards a Posthuman Condition
What are bacteria?
Planet of the Bacteria
Introductory Video Lectures in Biology[http://videolectures.net/mit7012f04_introduction_biology/
DNA from the beginning
Playing God for Fun and Profit
Design & Technology
Designer Bacteria may have a future in Fashion
Sunlight to Oil via Designer Bacteria
Loop.ph-Design Research Studio
Laughing in a sine curve- Abhishek Hazra[
Some interesting bio-design stuff by Brandon Ballangee and the likes. []
Student Book from IGEM 
Gel Electrophoresis 
Extracting DNA at home 
Harvard 2006: Explaining their process[]
Introduction to Biological Engineering Design 
Introduction to Synthetic Biology
Ibio Seminars 
Gel electrphoresis process
Primer on Synthetic Biology
DIY Synthetic Biology
Drinking straw gel electrophoresis
Open Gel Box 
BioBricks on Youtube 
General Design Links