ArtScience IGEM team
Contents
About
As amateurs/novices,we hope to bring our training in the arts and design as 'outsiders' and (hopefully) critical thinkers to synthetic biology, which, no doubt is a powerful tool and paradigm in looking at the life sciences. During the course of the workshop we hope to not only learn the tools and techniques of synthetic biology and come up with a piece of life which reflects our concerns but also use the process to engage with the political, ethical and cultural implications of Synthetic Biology.
People
Ideas
-bacteria that prevents corrosion
-Time keeper or clock
-Combustible bacteria
-Related to weather - smell of rain
-Bacteria that is resistant to Scientific Probing/Instruments
-Mirror of bacteria
-Buoyant bacteria
-Interactive bacteria(painting)
-Another creature made from bacteria
-Bacteria and sound
- Bacteria visualizations. (Both colour changes and 'choreographed' movement)
-Neuro transmitters- bacteria as sensors for emotions
-Glue
-Bacteria that detects cravings
-Magnetic bacteria
-Self mutating Bacteria
-Lie detector
-Bacteria creates an identity
-Bacteria becoming material on death
-Constructing a 'Bacterial Ecology'
-Bacteria that acts like oil
- A lubricant - A liquid that can withstand high temperatures
- Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
- Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.
Workshop
May 15th
-We discussed two Claire Pentacost Readings-Beyond Face and Critical Inventory of BioArt .
-The gist of the Pentacost readings were that artists work with the symbolic and that the Artist's consent to work and learn in public is important. -We also discussed the political and cultural implications of Scientific Authority. -We also looked at Tuur Van Balen's Urban Geography project -Most of the Ideas[see above] today, dealt with the use of bacteria as -a) A sensor or Reactor - (to Inputs,emotions,light..etc) -b) A Producer (of energy, proteins..etc) -c) A Material
-Is there Any way in which we can look at Bacteria from a purely non-symbiotic / non-anthropomorphic viewpoint? -Can we use our technological "progress" to give a non-selfish gift back to our ecological siblings?
-Replace financial transactions with Bacteria
May 16th
Here's some creatures we created using techno-scientific jargon and aesthetics:
IUpasana's Non Standard Regisitry of Names
Upasana's Ideas
Neha's Materialistica Destructica
Neha's Graph
Avni's actinodopaine
Amebitoni_Bacteria
Immuno Bacteria
Plastico Collecticus
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Id bacteria-Akash
Today's reading was called Speculative Fabulations for Technoculture's Generation by Donna Haraway.
-The article is primarily a review of the Australian artist Patricia Piccinini's work and a recapitulation of Haraway's philosophies .
-One of the things enduring about the reading was her appeal to "love" our creations, not in a tech.no- phillic sense but in a more nurturing and caring way.
May 17th
Hybrid Creatures From Mythology: Gallery
May 19th
We spent the day in NCBS picking up some standard biological techniques-Gel electrophoresis And looking at some of the microscopy equipment at NCBS.
Non-categorised images here
May 21st
We put down all the information that we had about learnt about geosmin. We then put down the various paths we could take in order to produce the results we wanted. This exercise cleared certain doubts we had, but also raised a lot of questions.
The Scientific Representation
The Artistic Representation
First Prototype of the Bacteria
May 22nd
Today's Reading:
May 25th
Presentation ideas- Bollywood sculpture?
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Its this big box. There is a projection of a window looking out into rain. All the paraphanalia can fit in the space below. fog machine?. The curtain goes all round.
How to read a scientific Paper by Mukund
1) Scan through the entire article quickly and find out what they are saying or trying to say.
2) Do not get lost in the references beyond 2 layers
How to create a gel electrophoresis chamber:
[[1]]
Today's Reading:
A synthetic Oscillatory network of transcriptional regulators
May 27th
http://openwetware.org/wiki/DIYbio:Notebook/Open_Gel_Box_2.0/RFC1
http://umassigem.blogspot.com/
http://diybio.org/2009/03/20/extract-dna-from-strawberries/
May 28th
Bio-Sculptures:
Avni's 'Chicken in a Kaleidoscope'
Neha's From a Spider
Spider-artist? A spider made to crawl through watercolor on white paper in a one-holed plastic box overnight.
An attempt to trace its movement in colors. Perhaps record a pattern?Would this be art?
Sandeep's Mustard seeds
May 29th
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Neha's 'TheArtScientist'-Page 1
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TheArtScientist-Page 2
May 30th
Aall DNA pictures have been uploaded. Take your pick here
1) Extracting DNA from Banana: <<Read here
2) Exracting DNA from Saliva:
Images of our DNA we extracted from saliva:
Neha's DNA
Krupakar's DNA
Dhruv's DNA
Sandeep's DNA
May 31st
Some interesting articles:
http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology
http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html
An interesting work:
http://www.edgarlissel.de/data/mnemosyne_II_01e.html (The photosensitive stripes are made of bacteria)
June 3rd
Registry of Parts Exercise
Sanya: Bacteria that indicates rise in and regulates body temperature.Media:Light.jpg
June 5th
Bacterial Transformation : The Process
The Bacterial Transformation Gallery
June 8th
Brief
The plasmids that contained the pBAD and Lysis genes that were prepared were extracted from the bacteria cultures. The unwanted protein around the DNA was then removed. After that the DNA was cleansed of any remaining salt. The plasmids were then cut at certain points such that only the required gene could be extracted. These cut parts were prepared for gel electrophoresis.
The Plasmid Preparation Miniprep Gallery
June 9th
For June 10th:
- (Lysis= K112808)
pBAD- promoter
Lysis-big construct- gene for cell lysis
Gel image of released pBAD(I0500), after digestion with EcoRI+PstI:
Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.
pBAD is digested with EcoRI +SPeI Lysis is digested with EcoRI + XbaI
A small interlink sequence between E and X will break open and spread as one band
For June 11th:
These bands will be kept overnight in 37 degrees Celsius.
Then these will be run through a preparative gel which takes about 3 hours.
The next step is to elute the released products which wold take 1.5 hours.
CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.
This enzyme will then be inactivated at 65 degree C.
This vector needs to be purified using column or chloroform
Other
http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm
It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.
http://igem.uwaterloo.ca/The_UW_iGEM_Team
Readings
Art and Politics
Claire Pentecost::Beyond Face[2]
Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt[3]
Donna Haraway::Speculative Fabulations for Technoculture's Generations[4]
Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life
Art
GeneAeshetics, The Art of Joe Davis[5]
Adam Zaretsky[6]
Patricia Piccinini[7]
Designer Bodies: Towards a Posthuman Condition[8]
Science
What are bacteria?[9]
Planet of the Bacteria[10]
Introductory Video Lectures in Biology[http://videolectures.net/mit7012f04_introduction_biology/
DNA from the beginning[11]
"'Ethics"'
Playing God for Fun and Profit[12]
Design & Technology
Urban BioGeography[13]
Designer Bacteria may have a future in Fashion[14]
Sunlight to Oil via Designer Bacteria[15]
Loop.ph-Design Research Studio[16]
Laughing in a sine curve- Abhishek Hazra[[17]
Some interesting bio-design stuff by Brandon Ballangee and the likes. [[18]]
Synthetic Biology:
Student Book from IGEM [19]
Gel Electrophoresis [20]
Extracting DNA at home [21]
Harvard 2006: Explaining their process[[22]]
The Synthetic Biology Comic[[23]] The .pdf version is here:[24]
Introduction to Biological Engineering Design [25]
Introduction to Synthetic Biology[26]
Ibio Seminars [27]
Gel electrphoresis process[28]
Primer on Synthetic Biology[29]
Drinking straw gel electrophoresis[30]
Open Gel Box [31]
BioBricks on Youtube [32]
Ginko BioWorks Guide to Synthetic Biology
General Design Links
http://psd.tutsplus.com/drawing/the-role-of-sketching-in-the-design-process/
