ArtScience IGEM team

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About

As amateurs/novices,we hope to bring our training in the arts and design as 'outsiders' and (hopefully) critical thinkers to synthetic biology, which, no doubt is a powerful tool and paradigm in looking at the life sciences. During the course of the workshop we hope to not only learn the tools and techniques of synthetic biology and come up with a piece of life which reflects our concerns but also use the process to engage with the political, ethical and cultural implications of Synthetic Biology.

People

Akash Hirosh

Dhruv Nawani

Nikhil Patil

Upasana Simha

Sandeep Mathew

Sanya Rai Gupta

Avni Sethi

Neha Bhat

Gautam Vishwanath

Krupakar Dhinakaran

Ideas

Click for a closer look

-bacteria that prevents corrosion

-Time keeper or clock

-Combustible bacteria

-Related to weather - smell of rain

-Bacteria that is resistant to Scientific Probing/Instruments

-Mirror of bacteria

-Buoyant bacteria

-Interactive bacteria(painting)

-Another creature made from bacteria

-Bacteria and sound

 - Bacteria visualizations. (Both colour changes and 'choreographed' movement)

-Neuro transmitters- bacteria as sensors for emotions

-Glue

-Bacteria that detects cravings

-Magnetic bacteria

-Self mutating Bacteria

-Lie detector

-Bacteria creates an identity

-Bacteria becoming material on death

-Constructing a 'Bacterial Ecology'

-Bacteria that acts like oil

 - A lubricant
 - A liquid that can withstand high temperatures
  • Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
  • Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.

Workshop

Week One

Week Two

Week Three

Week Four


June 9th

The June 9th Image Gallery

Images of the various results attained:

For June 14th:

  • (Lysis= K112808)

pBAD- promoter Lysis-big construct- gene for cell lysis Gel image of released pBAD(I0500), after digestion with EcoRI+PstI:

Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.

pBAD is digested with EcoRI +SPeI Lysis is digested with EcoRI + XbaI

A small interlink sequence between E and X will break open and spread as one band

For June 15th:

These bands will be kept overnight in 37 degrees Celsius.

Then these will be run through a preparative gel which takes about 3 hours.

The next step is to elute the released products which wold take 1.5 hours.

CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.

This enzyme will then be inactivated at 65 degree C.

This vector needs to be purified using column or chloroform

June 16th

Please look up this link before Monday. Special attention to restriction enzymes while you are at it! http://partsregistry.org/Assembly:Standard_assembly

Other

http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm

It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.

http://igem.uwaterloo.ca/The_UW_iGEM_Team

The IBB Pune Team's wiki [1]

Readings

Art and Politics

Claire Pentecost::Beyond Face[2]

Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt[3]

Donna Haraway::Speculative Fabulations for Technoculture's Generations[4]

Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life

Art

GeneAeshetics, The Art of Joe Davis[5]

Adam Zaretsky[6]

Patricia Piccinini[7]

Designer Bodies: Towards a Posthuman Condition[8]

Science

What are bacteria?[9]

Planet of the Bacteria[10]

Introductory Video Lectures in Biology[http://videolectures.net/mit7012f04_introduction_biology/

DNA from the beginning[11]

"'Ethics"'

Playing God for Fun and Profit[12]


Design & Technology

Urban BioGeography[13]

Designer Bacteria may have a future in Fashion[14]

Sunlight to Oil via Designer Bacteria[15]

Loop.ph-Design Research Studio[16]

Laughing in a sine curve- Abhishek Hazra[[17]

Some interesting bio-design stuff by Brandon Ballangee and the likes. [[18]]

Synthetic Biology:

Student Book from IGEM [19]

Gel Electrophoresis [20]

Extracting DNA at home [21]

Harvard 2006: Explaining their process[[22]]

The Synthetic Biology Comic[[23]] The .pdf version is here:[24]

Introduction to Biological Engineering Design [25]

Introduction to Synthetic Biology[26]

Ibio Seminars [27]

Gel electrphoresis process[28]

Primer on Synthetic Biology[29]

DIY Synthetic Biology[30]

Drinking straw gel electrophoresis

Drinking straw gel electrophoresis[31]

Open Gel Box [32]

BioBricks on Youtube [33]

Ginko BioWorks Guide to Synthetic Biology

DIY Biology Webcast

DIYBio Model Organisms

General Design Links

http://psd.tutsplus.com/drawing/the-role-of-sketching-in-the-design-process/

http://www.wallwisher.com/wall/iGemSrishti