Difference between revisions of "ArtScience IGEM team"

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(Workshop)
(May 17th)
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[[The May 17th Notebook]]
 
[[The May 17th Notebook]]
 
*Mukund
 
 
*People at NCBS-study
 
 
-Molecules
 
-MacroMolecules
 
-Cells
 
-Tissues
 
-Organs(very few people in NCBS study organs) Organs on their own might themselves not be Intersting
 
-Body
 
-Populations(Families, Groups of Individuals- Populations in really large Scales)
 
-Ancient DNA.(Samples from fossils)
 
 
 
'''Process'''
 
 
Powders
 
cutting DNA, Sequencing DNA
 
Machines: Microscopes, Influencing movement of molecules
 
Electrophysiology- Studying Neurons
 
 
DNA sequencing is very slow, so nothing much seems to be happening. To truely understand this, you need to go through an entire excercise.
 
 
 
'''Microscope'''
 
 
-4 people
 
 
'''DNA experiment'''
 
 
-5 people
 
 
Everybody wants to do both and then we can switch
 
 
Tomorrow at about 10 in the morning;we go through microscopy and half a round of DNA and on wednesday ,we finish the DNA experiment.
 
 
For the last 10 years the image is acquired on film or CCD chips once the image is done
 
we look at the digital image and we can look at the file and then extract the images.
 
 
Use MATLAB for image processing.
 
 
 
What are/were the big questions in Biology?
 
 
-Evolution
 
-Cell structure? (became important after the microscope).
 
-Before darwin it was about classifications and taxonomy.
 
-traits of offspring-how this information was transmitted.
 
-Homunculus-babies grow into bigger babies.
 
 
-Where are these instructions stored?
 
  studying diseases-they saw aberrations in chromosomes
 
  studying chromosomes during meiosis
 
 
 
In the 40s the big question was
 
-How does DNA store and transmit information
 
-DNA contains information ATCG:
 
-Allows you to replicate the information.
 
 
Hargobind Khurana figured out -
 
-How to read the information
 
-the series of letters help you make a protien-insulin
 
 
Since the 40,50's the questions haven't changed that much:
 
 
-If you give me the full genome of the organism- I have no idea what that means-I can guess what protiens it produces...
 
How does an animal form from the genome? No idea.
 
How signals in cells work? No Idea.
 
Can you make life from scratch? No idea.
 
 
 
Today in factories, we can synthesize every molecule in a cell but we cannot synthesize a cell
 
 
We can synthezise a virus(not a living thing)
 
 
We cannot make a cell (?)
 
 
How does the brain work?
 
 
Cells have a property of self-organisation.
 
 
Cells don't need to make themselves from scratch-It happened once in history
 
 
 
Mukund works with E-Coli . Ecoli has been studied for about 100 years, we know almost everything about it( genes, proteins, shape and structure)
 
*E coli has 4000 genes and can make 4000 different protiens
 
*The length of its genes is 4 million base pairs
 
*you need atleast 3 letters to make an amino acid
 
 
*It however only makes a 1000 protiens
 
 
There's something called Junk DNA, which is primarily misunderstood
 
 
 
--Switch--->Controller---->genes------>
 
 
We take genes and the switches that regulate them and make these circuit
 
 
*Make a DNA(switch) Gene(switch) DNA(switch)
 
 
*Then inject this into a cell
 
 
*The cell runs the program.
 
 
Why not just have a bunch of Genes? Why have a network?
 
 
Cell needs to process information.
 
Can we make a switch from scratch that responds only when the sugar is high AND temperature is high?
 
 
What else can switches do that genes cannot do:
 
 
*There are emergent properties
 
*the collection of oscillator
 
*memory
 
*positive feedback
 
 
Also understanding real cells:
 
 
Building instrumentation
 
 
Making cells from scratch.
 
  
 
=== '''May 19th''' ===
 
=== '''May 19th''' ===

Revision as of 08:11, 22 May 2009

Igemsrishtilogo.png


People

Akash Hirosh

Dhruv Nawani

Nikhil Patil

Upasana Simha

Sandeep Mathew

Sanya Rai Gupta

Avni Sethi

Neha Bhat

Gautam Vishwanath

Krupakar Dhinakaran

Ideas

-bacteria that prevents corrosion

-Time keeper or clock

-Combustible bacteria

-Related to weather - smell of rain

-Bacteria that is resistant to Scientific Probing/Instruments

-Mirror of bacteria

-Buoyant bacteria

-Interactive bacteria(painting)

-Another creature made from bacteria

-Bacteria and sound

 - Bacteria visualizations. (Both colour changes and 'choreographed' movement)

-Neuro transmitters- bacteria as sensors for emotions

-Glue

-Bacteria that detects cravings

-Magnetic bacteria

-Self mutating Bacteria

-Lie detector

-Bacteria creates an identity

-Bacteria becoming material on death

-Constructing a 'Bacterial Ecology'

-Bacteria that acts like oil

 - A lubricant
 - A liquid that can withstand high temperatures
  • Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
  • Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.

Workshop

May 15th

-We discussed two Claire Pentacost Readings-Beyond Face and Critical Inventory of BioArt .

-The gist of the Pentacost readings were that artists work with the symbolic and that the Artist's consent to work and learn in public is important.
-We also discussed the political and cultural implications of Scientific Authority.
-We also looked at Tuur Van Balen's Urban Geography project
-Most of the Ideas[see above] today, dealt with the use of bacteria as 
   -a) A sensor or Reactor - (to Inputs,emotions,light..etc)
    -b) A Producer (of energy, proteins..etc)
     -c) A Material

-Is there Any way in which we can look at Bacteria from a purely non-symbiotic / non-anthropomorphic viewpoint? -Can we use our technological "progress" to give a non-selfish gift back to our ecological siblings?

-Replace financial transactions with Bacteria

May 16th

Here's some creatures we created using techno-scientific jargon and aesthetics:


Today's reading was called Speculative Fabulations for Technoculture's Generation by Donna Haraway.

-The article is primarily a review of the Australian artist Patricia Piccinini's work and a recapitulation of Haraway's philosophies .

-One of the things enduring about the reading was her appeal to "love" our creations, not in a tech.no- phillic sense but in a more nurturing and caring way.

May 17th

Hybrid creatures from mythology:

The May 17th Notebook

May 19th

We spent the day in NCBS picking up some standard biological techniques-Gel electrophoresis And looking at some of the microscopy equipment at NCBS.




May 21th

We put down all the information that we had about learnt about geosmin. We then put down the various paths we could take in order to produce the results we wanted. This exercise cleared certain doubts we had, but also raised a lot of questions.

First Prototype of the Bacteria

Readings

Art and Politics

Claire Pentecost::Beyond Face[1]

Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt[2]

Donna Haraway::Speculative Fabulations for Technoculture's Generations[3]

Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life

Art

GeneAeshetics, The Art of Joe Davis[4]

Adam Zaretsky[5]

Patricia Piccinini[6]

Designer Bodies: Towards a Posthuman Condition[7]

Science

What are bacteria?[8]

Planet of the Bacteria[9]

Introductory Video Lectures in Biology[10]

Design & Technology

Urban BioGeography[11]

Designer Bacteria may have a future in Fashion[12]

Sunlight to Oil via Designer Bacteria[13]

Loop.ph-Design Research Studio[14]

Laughing in a sine curve- Abhishek Hazra[[15]

Some interesting bio-design stuff by Brandon Ballangee and the likes. [[16]]

Synthetic Biology:

Gel Electrophoresis [17]

Extracting DNA at home [18]

Harvard 2006: Explaining their process[[19]]

The Synthetic Biology Comic[[20]] The .pdf version is here:[21]

Introduction to Biological Engineering Design [22]

Introduction to Synthetic Biology[23]

Ibio Seminars [24]


General Design Links

http://psd.tutsplus.com/drawing/the-role-of-sketching-in-the-design-process/

http://www.wallwisher.com/wall/iGemSrishti