Difference between revisions of "ArtScience IGEM team"

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===June 9th===
===June 9th===

Revision as of 17:41, 9 June 2009



Akash Hirosh

Dhruv Nawani

Nikhil Patil

Upasana Simha

Sandeep Mathew

Sanya Rai Gupta

Avni Sethi

Neha Bhat

Gautam Vishwanath

Krupakar Dhinakaran


Click for a closer look

-bacteria that prevents corrosion

-Time keeper or clock

-Combustible bacteria

-Related to weather - smell of rain

-Bacteria that is resistant to Scientific Probing/Instruments

-Mirror of bacteria

-Buoyant bacteria

-Interactive bacteria(painting)

-Another creature made from bacteria

-Bacteria and sound

 - Bacteria visualizations. (Both colour changes and 'choreographed' movement)

-Neuro transmitters- bacteria as sensors for emotions


-Bacteria that detects cravings

-Magnetic bacteria

-Self mutating Bacteria

-Lie detector

-Bacteria creates an identity

-Bacteria becoming material on death

-Constructing a 'Bacterial Ecology'

-Bacteria that acts like oil

 - A lubricant
 - A liquid that can withstand high temperatures
  • Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
  • Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.


May 15th

-We discussed two Claire Pentacost Readings-Beyond Face and Critical Inventory of BioArt .

-The gist of the Pentacost readings were that artists work with the symbolic and that the Artist's consent to work and learn in public is important.
-We also discussed the political and cultural implications of Scientific Authority.
-We also looked at Tuur Van Balen's Urban Geography project
-Most of the Ideas[see above] today, dealt with the use of bacteria as 
   -a) A sensor or Reactor - (to Inputs,emotions,light..etc)
    -b) A Producer (of energy, proteins..etc)
     -c) A Material

-Is there Any way in which we can look at Bacteria from a purely non-symbiotic / non-anthropomorphic viewpoint? -Can we use our technological "progress" to give a non-selfish gift back to our ecological siblings?

-Replace financial transactions with Bacteria

May 16th

Here's some creatures we created using techno-scientific jargon and aesthetics:

Today's reading was called Speculative Fabulations for Technoculture's Generation by Donna Haraway.

-The article is primarily a review of the Australian artist Patricia Piccinini's work and a recapitulation of Haraway's philosophies .

-One of the things enduring about the reading was her appeal to "love" our creations, not in a tech.no- phillic sense but in a more nurturing and caring way.

May 17th

Hybrid creatures from mythology:

May 19th

We spent the day in NCBS picking up some standard biological techniques-Gel electrophoresis And looking at some of the microscopy equipment at NCBS.

Some images of our day at NCBS:

Non-categorised images here

May 21st

We put down all the information that we had about learnt about geosmin. We then put down the various paths we could take in order to produce the results we wanted. This exercise cleared certain doubts we had, but also raised a lot of questions.

First Prototype of the Bacteria

May 22nd

Today's Reading:


Some basics of Gene Expression and production of Protiens and Enzymes by Gene's

May 25th

May 23rd-May 25th

Presentation ideas- Bollywood sculpture?

How to read a scientific Paper by Mukund

Scan through the entire article quickly and find out what they are saying or trying to say Do not get lost in the references beyond 2 layers

how to create a gel electrophoresis chamber.[[1]]

Today's Reading:

A synthetic Oscillatory network of transcriptional regulators

May 27th




May 28th


Avni's 'Chicken in a Kaleidoscope'

Neha's From a Spider

Sandeep's Mustard seeds

May 29th

May 30th

(all DNA pictures have been uploaded. Take your pick here. (Not like you have a lot of options or anything..))

Extracting DNA from Banana:

What you need

  1. 3 glass or clear plastic cups
  2. A cup of water
  3. A banana
  4. A spoon
  5. An eyedropper
  6. A strainer
  7. A funnel
  8. A toothpick
  9. A tablespoon of salt
  10. 3 Tablespoons of liquid detergent containing Sodium Laurel Sulphate (look at back of the bottle)
  11. A third of a cup of rubbing alcohol

What you need to do

  1. Put the rubbing alcohol in the freezer to chill it.
  2. Mash the banana up with the spoon.
  3. Strain the pulp through the funnel into one of the empty glasses (You might need some water to help it through.)
  4. In another glass stir the detergent and salt together.
  5. Mix the banana pulp and the salty detergent together for about a minute. Try to avoid bubbles.
  6. When it settles, gently pour the alcohol down the side of the cup over the back end of the spoon. The alcohol should form a layer over the mixture.
  7. Soon, you'll see tiny white strands of millions DNA gathering between the two layers.
  8. Pick them out with the toothpick. They should be white, whiter than the rest of the banana pulp. Admire!

Exracting DNA from Saliva:

Images of our DNA we extracted from saliva:

May 31st

Some interesting articles I came across.


http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html - I haven't read this one properly yet!! But the topic seems interesting and relevant...

An interesting work i found while surfing- http://www.edgarlissel.de/data/mnemosyne_II_01e.html the photosensitive stripes are made of bacteria

June 3rd

Registry of Parts Exercise

Sanya: Bacteria that indicates rise in and regulates body temperature.Media:Light.jpg


Neha's Bacterial Jewellery

June 5th

Bacterial Transformation : The Process

June 8th

The plasmids that contained the pBAD and Lysis genes that were prepared were extracted from the bacteria cultures. The unwanted protein around the DNA was then removed. After that the DNA was cleansed of any remaining salt. The plasmids were then cut at certain points such that only the required gene could be extracted. These cut parts were prepared for gel electrophoresis.

The process

June 9th

Agarose gel electrophoresis image of digested K112808: Gelimage090609.png To do for tomorrow:- (Lysis= K112808)

pBAD- promoter Lysis-big construct- gene for cell lysis

Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.

pBAD is digested with EcoRI +SPeI Lysis is digested with EcoRI + XbaI

A small interlink sequence between E and X will break open and spread as one band

To do for day after tomorrow: -

These bands will be kept overnight in 37 degrees Celsius.

Then these will be run through a preparative gel which takes about 3 hours.

The next step is to elute the released products which wold take 1.5 hours.

CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.

This enzyme will then be inactivated at 65 degree C.

This vector needs to be purified using column or chloroform

Other Teams

It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.



The iGEM India 2009 Google group[2]

Art and Politics

Claire Pentecost::Beyond Face[3]

Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt[4]

Donna Haraway::Speculative Fabulations for Technoculture's Generations[5]

Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life


GeneAeshetics, The Art of Joe Davis[6]

Adam Zaretsky[7]

Patricia Piccinini[8]

Designer Bodies: Towards a Posthuman Condition[9]


What are bacteria?[10]

Planet of the Bacteria[11]

Introductory Video Lectures in Biology[http://videolectures.net/mit7012f04_introduction_biology/

DNA from the beginning[12]


Playing God for Fun and Profit[13]

Design & Technology

Urban BioGeography[14]

Designer Bacteria may have a future in Fashion[15]

Sunlight to Oil via Designer Bacteria[16]

Loop.ph-Design Research Studio[17]

Laughing in a sine curve- Abhishek Hazra[[18]

Some interesting bio-design stuff by Brandon Ballangee and the likes. [[19]]

Synthetic Biology:

Student Book from IGEM [20]

Gel Electrophoresis [21]

Extracting DNA at home [22]

Harvard 2006: Explaining their process[[23]]

The Synthetic Biology Comic[[24]] The .pdf version is here:[25]

Introduction to Biological Engineering Design [26]

Introduction to Synthetic Biology[27]

Ibio Seminars [28]

Gel electrphoresis process[29]

Primer on Synthetic Biology[30]

Drinking straw gel electrophoresis

Drinking straw gel electrophoresis[31]

Open Gel Box [32]

BioBricks on Youtube [33]

Ginko BioWorks Guide to Synthetic Biology

DIY Biology Webcast

DIYBio Model Organisms

General Design Links