Difference between revisions of "ArtScience IGEM team"

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http://igem.uwaterloo.ca/The_UW_iGEM_Team
 
http://igem.uwaterloo.ca/The_UW_iGEM_Team
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[http://http://2009.igem.org/Team:IBB_Pune#|The IBB Pune Team's wiki]
  
 
== Readings ==
 
== Readings ==

Revision as of 07:41, 13 June 2009

Igemsrishtilogo.png


About

As amateurs/novices,we hope to bring our training in the arts and design as 'outsiders' and (hopefully) critical thinkers to synthetic biology, which, no doubt is a powerful tool and paradigm in looking at the life sciences. During the course of the workshop we hope to not only learn the tools and techniques of synthetic biology and come up with a piece of life which reflects our concerns but also use the process to engage with the political, ethical and cultural implications of Synthetic Biology.

People

Akash Hirosh

Dhruv Nawani

Nikhil Patil

Upasana Simha

Sandeep Mathew

Sanya Rai Gupta

Avni Sethi

Neha Bhat

Gautam Vishwanath

Krupakar Dhinakaran

Ideas

Click for a closer look

-bacteria that prevents corrosion

-Time keeper or clock

-Combustible bacteria

-Related to weather - smell of rain

-Bacteria that is resistant to Scientific Probing/Instruments

-Mirror of bacteria

-Buoyant bacteria

-Interactive bacteria(painting)

-Another creature made from bacteria

-Bacteria and sound

 - Bacteria visualizations. (Both colour changes and 'choreographed' movement)

-Neuro transmitters- bacteria as sensors for emotions

-Glue

-Bacteria that detects cravings

-Magnetic bacteria

-Self mutating Bacteria

-Lie detector

-Bacteria creates an identity

-Bacteria becoming material on death

-Constructing a 'Bacterial Ecology'

-Bacteria that acts like oil

 - A lubricant
 - A liquid that can withstand high temperatures
  • Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
  • Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.

Workshop

May 15th

-We discussed two Claire Pentacost Readings-Beyond Face and Critical Inventory of BioArt .

-The gist of the Pentacost readings were that artists work with the symbolic and that the Artist's consent to work and learn in public is important.
-We also discussed the political and cultural implications of Scientific Authority.
-We also looked at Tuur Van Balen's Urban Geography project
-Most of the Ideas[see above] today, dealt with the use of bacteria as 
   -a) A sensor or Reactor - (to Inputs,emotions,light..etc)
    -b) A Producer (of energy, proteins..etc)
     -c) A Material

-Is there Any way in which we can look at Bacteria from a purely non-symbiotic / non-anthropomorphic viewpoint? -Can we use our technological "progress" to give a non-selfish gift back to our ecological siblings?

-Replace financial transactions with Bacteria

May 16th

Here's some creatures we created using techno-scientific jargon and aesthetics:


Today's reading was called Speculative Fabulations for Technoculture's Generation by Donna Haraway.

-The article is primarily a review of the Australian artist Patricia Piccinini's work and a recapitulation of Haraway's philosophies .

-One of the things enduring about the reading was her appeal to "love" our creations, not in a tech.no- phillic sense but in a more nurturing and caring way.

May 17th

Hybrid Creatures From Mythology: Gallery

May 19th

We spent the day in NCBS picking up some standard biological techniques-Gel electrophoresis And looking at some of the microscopy equipment at NCBS.


Some Images Of Our Day

Non-categorised images here

May 21st

We put down all the information that we had about learnt about geosmin. We then put down the various paths we could take in order to produce the results we wanted. This exercise cleared certain doubts we had, but also raised a lot of questions.

First Prototype of the Bacteria

May 22nd

Today's Reading:

Some basics of Gene Expression and production of Proteins and Enzymes by Genes

May 25th

Presentation ideas- Bollywood sculpture?


How to read a scientific Paper by Mukund

1) Scan through the entire article quickly and find out what they are saying or trying to say.

2) Do not get lost in the references beyond 2 layers


How to create a gel electrophoresis chamber:

[[1]]


Today's Reading:

A synthetic Oscillatory network of transcriptional regulators


May 27th

http://openwetware.org/wiki/DIYbio:Notebook/Open_Gel_Box_2.0/RFC1

http://umassigem.blogspot.com/

http://diybio.org/2009/03/20/extract-dna-from-strawberries/


May 28th

Bio-Sculptures:

Avni's 'Chicken in a Kaleidoscope'

Neha's From a Spider


Sandeep's Mustard seeds

May 29th


May 30th

All DNA pictures have been uploaded. Take your pick here

1) Extracting DNA from Banana: <<Read here

2) Exracting DNA from Saliva:

Images of our DNA we extracted from saliva:

May 31st

Some interesting articles:

http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology

http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html

An interesting work:

http://www.edgarlissel.de/data/mnemosyne_II_01e.html (The photosensitive stripes are made of bacteria)

June 3rd

Registry of Parts Exercise

Sanya: Bacteria that indicates rise in and regulates body temperature.Media:Light.jpg

Upasana

Neha's Bacterial Jewellery

June 5th

Beginning laboratory procedures:

1) Bacterial Transformation : The Process | The Bacterial Transformation Gallery

2) Preparing the Plasmid (minprep)

3) Digesting the DNA

4) Running the gel (Gel Electrophoresis)

5) Elution

June 8th

Preparing the Plasmids

A Brief of what we did:

The plasmids that contained the pBAD and Lysis genes that were prepared were extracted from the bacteria cultures. The unwanted protein around the DNA was then removed. After that the DNA was cleansed of any remaining salt. The plasmids were then cut at certain points such that only the required gene could be extracted. These cut parts were prepared for gel electrophoresis.

The process | The Plasmid Preparation Miniprep Gallery

June 9th

The June 9th Image Gallery

Images of the various results attained:

For June 10th:

  • (Lysis= K112808)

pBAD- promoter Lysis-big construct- gene for cell lysis Gel image of released pBAD(I0500), after digestion with EcoRI+PstI:

Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.

pBAD is digested with EcoRI +SPeI Lysis is digested with EcoRI + XbaI

A small interlink sequence between E and X will break open and spread as one band

For June 11th:

These bands will be kept overnight in 37 degrees Celsius.

Then these will be run through a preparative gel which takes about 3 hours.

The next step is to elute the released products which wold take 1.5 hours.

CIP treatment will be done [Calf Intestine Phosphate] This treatment is given to only the vector of Lysis (Process will take 1.5 hour) This is done so that the vector doesn't self ligate.

This enzyme will then be inactivated at 65 degree C.

This vector needs to be purified using column or chloroform

June 12th

Please look up this link before Monday. Special attention to restriction enzymes while you are at it! http://partsregistry.org/Assembly:Standard_assembly

Other

http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm

It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.

http://igem.uwaterloo.ca/The_UW_iGEM_Team

IBB Pune Team's wiki

Readings

Art and Politics

Claire Pentecost::Beyond Face[2]

Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt[3]

Donna Haraway::Speculative Fabulations for Technoculture's Generations[4]

Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life

Art

GeneAeshetics, The Art of Joe Davis[5]

Adam Zaretsky[6]

Patricia Piccinini[7]

Designer Bodies: Towards a Posthuman Condition[8]

Science

What are bacteria?[9]

Planet of the Bacteria[10]

Introductory Video Lectures in Biology[http://videolectures.net/mit7012f04_introduction_biology/

DNA from the beginning[11]

"'Ethics"'

Playing God for Fun and Profit[12]


Design & Technology

Urban BioGeography[13]

Designer Bacteria may have a future in Fashion[14]

Sunlight to Oil via Designer Bacteria[15]

Loop.ph-Design Research Studio[16]

Laughing in a sine curve- Abhishek Hazra[[17]

Some interesting bio-design stuff by Brandon Ballangee and the likes. [[18]]

Synthetic Biology:

Student Book from IGEM [19]

Gel Electrophoresis [20]

Extracting DNA at home [21]

Harvard 2006: Explaining their process[[22]]

The Synthetic Biology Comic[[23]] The .pdf version is here:[24]

Introduction to Biological Engineering Design [25]

Introduction to Synthetic Biology[26]

Ibio Seminars [27]

Gel electrphoresis process[28]

Primer on Synthetic Biology[29]

DIY Synthetic Biology[30]

Drinking straw gel electrophoresis

Drinking straw gel electrophoresis[31]

Open Gel Box [32]

BioBricks on Youtube [33]

Ginko BioWorks Guide to Synthetic Biology

DIY Biology Webcast

DIYBio Model Organisms

General Design Links

http://psd.tutsplus.com/drawing/the-role-of-sketching-in-the-design-process/

http://www.wallwisher.com/wall/iGemSrishti