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− | [[File:Igemsrishtilogo.png]]
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| + | [[File:hackteria banner1.png]] |
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| == About == | | == About == |
− | As amateurs/novices,we hope to bring our training in the arts and
| + | We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. This approach allowed us to engage in constructive discussions with scientists working in the field. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. We have expressed this gene in different strains of E. coli, under control of the pTrc promoter present in the pTrc99a vector. Geosmin is responsible for producing the earthy smell that occurs in the air when rain falls after a dry spell of weather. |
− | design as 'outsiders' and (hopefully) critical thinkers to synthetic | |
− | biology, which, no doubt is a powerful tool and paradigm in looking at | |
− | the life sciences.
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− | During the course of the workshop we hope to not only learn the tools
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− | and techniques of synthetic biology and come up with a piece of life | |
− | which reflects our concerns but also use the process to engage with | |
− | the political, ethical and cultural implications of Synthetic Biology. | |
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| == People == | | == People == |
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| [[File:Whiteboard.jpg|820px|none|Click for a closer look]] | | [[File:Whiteboard.jpg|820px|none|Click for a closer look]] |
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− | -bacteria that prevents corrosion | + | Major Outcomes - |
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− | -Time keeper or clock
| + | * [[Ideas for Bacteria.]] |
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− | -Combustible bacteria
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− | -Related to weather - smell of rain
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− | -Bacteria that is resistant to Scientific Probing/Instruments
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− | -Mirror of bacteria
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− | -Buoyant bacteria
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− | -Interactive bacteria(painting)
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− | -Another creature made from bacteria
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− | -Bacteria and sound
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− | - Bacteria visualizations. (Both colour changes and 'choreographed' movement)
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− | -Neuro transmitters- bacteria as sensors for emotions
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− | -Glue
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− | -Bacteria that detects cravings
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− | -Magnetic bacteria
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− | -Self mutating Bacteria
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− | -Lie detector
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− | -Bacteria creates an identity
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− | -Bacteria becoming material on death
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− | -Constructing a 'Bacterial Ecology'
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− | -Bacteria that acts like oil
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− | - A lubricant
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− | - A liquid that can withstand high temperatures
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| * Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure. | | * Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure. |
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| == Workshop == | | == Workshop == |
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− | [[Week One]]
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− | [[Week Two]]
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− | === May 30th ===
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− | All DNA pictures have been uploaded. Take your pick [[Special:ListFiles|here]]
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− | 1) '''Extracting DNA from Banana:''' <<[[Read here]]
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− | 2) '''Exracting DNA from Saliva:'''
| + | {| border="0" cellspacing="0" cellpadding="4" align="center" |
| + | ![[Week One]] |
| + | ![[Week Two]] |
| + | ![[Week Three]] |
| + | ![[Week Four]] |
| + | |- |
| + | ![[Week Five]] |
| + | ![[Week Six]] |
| + | ![[Week Seven]] |
| + | ![[Week Eight]] |
| + | |} |
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− | '''Images of our DNA we extracted from saliva:'''
| + | <br/> |
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− | <gallery>
| + | === Other === |
− | File:NehasDNA.JPG|Neha's DNA
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− | File:KrupakarsDNA.JPG|Krupakar's DNA
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− | File:DhruvsDNA.JPG|Dhruv's DNA
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− | File:SandeepsDNA.JPG|Sandeep's DNA
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− | </gallery>
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− | | |
− | ===May 31st===
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− | Some interesting articles:
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− | http://www.thenewatlantis.com/publications/the-promise-and-perils-of-synthetic-biology
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− | http://www.nature.com/msb/journal/v2/n1/full/msb4100104.html
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− | An interesting work:
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− | http://www.edgarlissel.de/data/mnemosyne_II_01e.html
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− | (The photosensitive stripes are made of bacteria)
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− | | |
− | ===June 3rd===
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− | '''''Registry of Parts Exercise'''''
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− | Sanya: Bacteria that indicates rise in and regulates body temperature.[[Media:Light.jpg]]
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− | [[Upasana]]
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− | [[Neha's Bacterial Jewellery]]
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− | | |
− | ===June 5th===
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− | '''Beginning laboratory procedures:'''
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− | 1) [[Bacterial Transformation : The Process]] | [[The Bacterial Transformation Gallery]]
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− | 2) Preparing the Plasmid (minprep)
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− | 3) Digesting the DNA
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− | 4) Running the gel (Gel Electrophoresis)
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− | 5) Elution
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− | | |
− | ===June 8th===
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− | '''''[[A Brief Description of What We Will Do]]'''''
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− | [[Preparing the pBAD and Lysis plasmids for gel electrophoresis|The Plasmid preparation process]] | [[The Plasmid Preparation Miniprep Gallery]]
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− | ''' A Brief of what we did: ''' <br/>
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− | The plasmids that contained the pBAD and Lysis genes that were prepared were extracted from the bacteria cultures. The unwanted protein around the DNA was then removed. After that the DNA was cleansed of any remaining salt. The plasmids were then cut at certain points such that only the required gene could be extracted. These cut parts were prepared for gel electrophoresis.
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− | ===June 9th===
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− | [[The June 9th Image Gallery]]
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− | Images of the various results attained:
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− | <gallery>
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− | File:Gelimage090609.png
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− | File:Not working.jpg
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− | File:PBAD-insert f.png
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− | </gallery>
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− | | |
− | '''For June 14th:'''
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− | *(Lysis= K112808)
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− | pBAD- promoter
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− | Lysis-big construct- gene for cell lysis
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− | Gel image of released pBAD(I0500), after digestion with EcoRI+PstI:
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− | Each pBAd and Lysis has 4 restriction enzymes E, X,S, P.
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− | pBAD is digested with EcoRI +SPeI
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− | Lysis is digested with EcoRI + XbaI
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− | A small interlink sequence between E and X will break open and spread as one band
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− | '''For June 15th:'''
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− | These bands will be kept overnight in 37 degrees Celsius.
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− | Then these will be run through a preparative gel which takes about 3 hours.
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− | The next step is to [[elute the released products]] which wold take 1.5 hours.
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− |
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− | CIP treatment will be done
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− | [Calf Intestine Phosphate]
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− | This treatment is given to only the vector of Lysis (Process will take 1.5 hour)
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− | This is done so that the vector doesn't self ligate.
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− | This enzyme will then be inactivated at 65 degree C.
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− | This vector needs to be purified using column or chloroform
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− | '''June 16th'''
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− | Please look up this link before Monday. Special attention to restriction enzymes while you are at it!
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− | http://partsregistry.org/Assembly:Standard_assembly
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− | ===Other === | |
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| http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm | | http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm |
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| The IBB Pune Team's wiki | | The IBB Pune Team's wiki |
| [http://2009.igem.org/Team:IBB_Pune#] | | [http://2009.igem.org/Team:IBB_Pune#] |
| + | |
| + | [http://2008.igem.org/Team:NYMU-Taipei NYMU-Taipei's 2008 Wiki] |
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| == Readings == | | == Readings == |
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| + | '''[[Art and Politics]]''' |
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| + | '''[[Art]]''' |
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− | '''Art and Politics''' | + | '''[[Science]]''' |
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− | Claire Pentecost::Beyond Face[http://www.clairepentecost.org/beyond_face.htm]
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− | Claire Pentecost::Outfitting the Laboratory of the Symbolic: Towards a Critical Inventory of BioArt[http://www.clairepentecost.org/lab%20of%20symbolic_text.htm]
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− | Donna Haraway::Speculative Fabulations for Technoculture's Generations[http://www.patriciapiccinini.net/essay.php?id=30]
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− | Oron Catts, Ionat Zurr::The ethics of experiential engagement with the manipulation of life
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− | '''Art'''
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− | GeneAeshetics, The Art of Joe Davis[http://www.clondiag.com/frame.php?page=/art/joe.davis/index.php?docid=0]
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− | Adam Zaretsky[http://www.emutagen.com/]
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− | Patricia Piccinini[http://www.patriciapiccinini.net/]
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− | Designer Bodies: Towards a Posthuman Condition[http://www.a-r-c.org.uk/db/about.html]
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− | '''Science'''
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− | What are bacteria?[http://www.disknet.com/indiana_biolab/b004.htm]
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− | Planet of the Bacteria[http://www.stephenjaygould.org/library/gould_bacteria.html]
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− | Introductory Video Lectures in Biology[http://videolectures.net/mit7012f04_introduction_biology/
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− | DNA from the beginning[http://www.dnaftb.org/22/concept/index.html]
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− | "'Ethics"'
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− | Playing God for Fun and Profit[http://radar.oreilly.com/2008/02/play-god-for-fun-and-profit-mo.html]
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− | '''Design & Technology'''
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− | Urban BioGeography[http://www.tuurvanbalen.com/projects/urbanbiogeography]
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− | Designer Bacteria may have a future in Fashion[http://www.nytimes.com/2001/08/19/style/designer-bacteria-may-have-a-future-in-fashion.html]
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− | Sunlight to Oil via Designer Bacteria[http://www.thinkgene.com/light-oil-with-bacteria/]
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− | Loop.ph-Design Research Studio[http://loop.ph/bin/view/Loop/WebHome]
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− | Laughing in a sine curve- Abhishek Hazra[[http://abhishekhazra.blogspot.com/2009/04/laughing-in-sine-curve.html]
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− | Some interesting bio-design stuff by Brandon Ballangee and the likes. [[http://io9.com/photogallery/biotechnique/1000502846]]
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− | '''Synthetic Biology:
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− | '''
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− | Student Book from IGEM [http://openwetware.org/images/f/f9/IGEM_Student_Book.pdf]
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− | Gel Electrophoresis [http://en.wikipedia.org/wiki/Gel_electrophoresis]
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− | Extracting DNA at home [http://nature.ca/genome/05/051/pdfs/DNAextract_e.pdf]
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− | Harvard 2006: Explaining their process[[http://parts2.mit.edu/wiki/index.php/Harvard_2006]]
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− | The Synthetic Biology Comic[[http://www.nature.com/nature/comics/syntheticbiologycomic/]] The .pdf version is here:[http://mit.edu/endy/www/scraps/comic/AiSB.vol1.pdf]
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− | Introduction to Biological Engineering Design [http://openwetware.org/wiki/20.20]
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− | Introduction to Synthetic Biology[http://openwetware.org/wiki/Intertech:iSB2008:Materials]
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− | Ibio Seminars [http://www.ibioseminars.org/]
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− | Gel electrphoresis process[http://web.utk.edu/~khughes/GEL/sld008.htm]
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− | Primer on Synthetic Biology[http://openwetware.org/images/3/3d/SB_Primer_100707.pdf]
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− | DIY Synthetic Biology[http://www.scribd.com/doc/14663730/DIY-Synthetic-Biology]
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− | [[File:Keiki-gel-1-225x300.jpg|150px|thumb|none|Drinking straw gel electrophoresis]]
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− | Drinking straw gel electrophoresis[http://maradydd.livejournal.com/417631.html]
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− | Open Gel Box [http://openwetware.org/wiki/DIYbio:Notebook/Open_Gel_Box_2.0]
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− | BioBricks on Youtube [http://www.youtube.com/user/BioBricksFoundation]
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− | [http://diybio.org/wp-content/uploads/2009/04/CodeCon09_SynthBio_tutorial_handout/appendix3%20-%20GinkgoBioworks%20guide%20to%20engineering%20biology.pdf Ginko BioWorks Guide to Synthetic Biology]
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− | [http://techtv.mit.edu/collections/mitmuseum/videos/1684-soap-box-do-it-yourself-biology DIY Biology Webcast]
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− | [http://groups.google.com/group/diybio/web/diybio-model-organisms DIYBio Model Organisms] | + | '''[[Ethics]]''' |
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− | '''General Design Links''' | + | '''[[Design & Technology]]''' |
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− | http://psd.tutsplus.com/drawing/the-role-of-sketching-in-the-design-process/
| + | '''[[Synthetic Biology]]''' |
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− | http://www.wallwisher.com/wall/iGemSrishti
| + | '''[[General Design Links]]''' |
We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. This approach allowed us to engage in constructive discussions with scientists working in the field. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. We have expressed this gene in different strains of E. coli, under control of the pTrc promoter present in the pTrc99a vector. Geosmin is responsible for producing the earthy smell that occurs in the air when rain falls after a dry spell of weather.
It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.