Algae Culture at Home

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Plankton.jpg

http://openlibrary.org/books/OL8553218M/Plankton_Culture_Manual

http://www.scientificamerican.com/article.cfm?id=how-to-rear-a-plankton-me

http://www.instructables.com/id/Simple-Algae-Home-CO2-Scrubber-Part-II-Culturing/?ALLSTEPS

Experimental Flat-Panel Photobioreactor:

http://arkfab.org/?cat=135

Grow-> extract -> distill to make biofuel:

http://www.ehow.com/how_5982439_use-algae-biodiesel-fuel.html


Algeator1.jpg

Algea incubator with arduino controlled CO2 pump, simple resistive heater and some led lightning

Algeator3.jpg Algeator2.jpg
fabbing the plexi parts in the FabLab / lights on

Algae Hacking at GaudiLabs (June 2012)

Picking Algae Cells / Patch Clamp

WebCamCellPicker.jpg
Setup using a CD-ROM reading head for XY micro-positioning of a small glas capillary. The coil head is controlled using an arduino. The coils are directely connected to the PWM output pins.

XYCellPicker.jpg
Picking head mounted on a XY-table for macro adjustments using the two stepper motors.

Clamp.jpg
Imgae of the capillary. String algae and single cell algae in size comparison. Positioning is very precise (+/- 5 um),aspiring the algae is not yet reliable.


PatchClamp Theory.jpg
Discussed some theory on patch clamping cells brought in by Ralf Weise.


We need the Chara Corallina algae with single cells as big a 2x20mm.

Chara Corallina.jpg
Let's go for algae hunting...

Algae Sample Collection


CollectingSamples.jpg
We go out with the pedalo on lake Lucern to best aproach the nice spots for collecting algae.
Took samples on various places and put them into the PET containers (got them from dumpster diving at main station).

SampleInspection.jpg
Back home, instpecting the sample using the webcam microscope.
Suprised about the very diverse aquatic communities.
Theory brought in by Sachiko:
"A biofilm is an aggregate of microorganisms in which cells adhere to each other on a surface."
Biofilm is also referred to as slime...

Algae Samples Inspection

AlgaeLog.jpg
AlgaeBoat.jpg
AlgaeFountain.jpg
AlgaePond.jpg
AlgaeBiger.jpg

Open Lab Session

Links: http://www.fao.org/DOCREP/003/W3732E/w3732e06.htm http://en.wikipedia.org/wiki/Biofilm