Difference between revisions of "Actinomycetes Tournament: Open antibiotics discovery"

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* [BioStrike workshop at Innoculab http://www.innoculab.org]
 
* [BioStrike workshop at Innoculab http://www.innoculab.org]
 
* [BioStrike Flickr http://www.flickr.com/groups/2394197@N23/]
 
* [BioStrike Flickr http://www.flickr.com/groups/2394197@N23/]
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* [BioStrike Zotero References https://www.zotero.org/groups/biostrike/items]
 
Bergey's Manual of Systematic Bacteriology - original edition [http://archive.org/details/bergeysmanualofd1957amer]<br>
 
Bergey's Manual of Systematic Bacteriology - original edition [http://archive.org/details/bergeysmanualofd1957amer]<br>
 
Who is who in the microbial world? [http://www2.nau.edu/~fpm/bio205/taxon1.html]<br><br>
 
Who is who in the microbial world? [http://www2.nau.edu/~fpm/bio205/taxon1.html]<br><br>
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Example of present research and protocol [http://www.bioinfopublication.org/files/articles/2_2_3_IJMR.pdf]: the best sites were non  agricultural wasteland alkaline soil and compost rich garden soil.
 
Example of present research and protocol [http://www.bioinfopublication.org/files/articles/2_2_3_IJMR.pdf]: the best sites were non  agricultural wasteland alkaline soil and compost rich garden soil.
 
Weird actinomycetes as symbionts with ants [http://www.biomedcentral.com/1741-7007/8/109/]
 
Weird actinomycetes as symbionts with ants [http://www.biomedcentral.com/1741-7007/8/109/]
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== Techniques ==
 
== Techniques ==
 
Agar diffusion test [http://en.wikipedia.org/wiki/Agar_diffusion_test]<br>
 
Agar diffusion test [http://en.wikipedia.org/wiki/Agar_diffusion_test]<br>

Revision as of 12:52, 30 October 2013

Game2013.png

Picture: Initial brainstorming with DIYbio Waag


We want to screen soil bacteria, such as Actinomyces, Streptomyces and Micromonospora for antibiotic properties. It is a global citizen science initiative started by the Waag Open Wetlab, which uses game elements (collecting and tournaments) to support open drug discovery of antibiotics. We will isolate actinomycetes from soil samples around the world and screen with Micrococcus luteus or similar microbe to determine their antibiotic properties. Images of inoculated plates will be shared online and based on their strengths (zones of growth inhibition) they will become part of a collection or initiate tournaments between the most successful examples. We will sequence the most powerful ones. We expect cooperation with various DIYbio organizations, microbiology departments and science enthusiasts.

EDITING IN PROGRESS!!
This is a wiki for documentation, sharing links and defining the protocol

Objectives

1. Improve, standardize and simplify the protocol [1] and its different phases: diluting a soil sample, inoculation on various agar media, incubate for a specified time, cross streaks with non pathogenic bacteria, optimal temperature for incubation, examining growth inhibition, drying or amplifying with various OSHW tools and sending to our potential scientific partner for sequencing.
2. Gamification of agar plates: growth inhibition circles in the plates will define various "collections" and the most powerful ones will take part in tournaments. Every participant will have a page with his collection and the most powerful strains will be featured on the main page. All data will be shared under CC for open science with database of GPS data and pictures of the sites where the soil samples were collected and soil bank.


Resources

The development of the project is documented on the following platforms:

Bergey's Manual of Systematic Bacteriology - original edition [2]
Who is who in the microbial world? [3]

What are Actinomycetes [4]
How to recognize them? Middle and and right side of this plate [5]

Antibiotics and Actinomycetes classic http://link.springer.com/article/10.1007/BF02861819
Nature of the substrate where you take the sample: those from decaying wood were the least active, whereas those obtained from the rhizosphere of growing plants were most active. At the same time another author cited there says that "high percentage of antagonistic forms which they found in soils from northern regions may be related to the sparser vegetation and to the greater proportion of time during which no plant development occur (scarcity issue? more competition?)" Example of present research and protocol [6]: the best sites were non agricultural wasteland alkaline soil and compost rich garden soil. Weird actinomycetes as symbionts with ants [7]

Techniques

Agar diffusion test [8]
We should use Open CFU. Article "OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects [9]

You will need

We will try to make this list modular, so that you can do each part independently.

Equipment for

  • Autoclave / pressure cooker
  • Incubator
  • Fridge
  • Balance

Consumable materials

  • Petri dishes / tupper ware
  • Agar / agar agar
  • LB complete medium
  • Bottles

Protocols

Example of a professional protocol [10]
Microbiology Tutorials [11]

Discussion

Big Pharma doesn't invest in new antibiotics, there is overuse in animals and humans, which will eventually create a drug resistant bacteria. [12]
Open Drug Discovery and Big Pharma [13]

DIYbio general - examples to follow

Biocoder [14]
Biohacker projects [15]
GetIt [16]
Biohacking [17]