A Brief Description of What We Will Do

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Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.

Step 3 - Pick a single colony.

Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.

Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA

Step 7 - Purifying the DNA