A Brief Description of What We Will Do
Step 1 - Taking dry DNA from wells
1. Making sure the plates are properly oriented, punch a hole through the foil cover using a pipette - into the well you want the DNA from. Don't remove foil cover since it might pose the threat of cross-contamination between wells.
2. Add 15uL of deionized water - diH2O Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Pick a single colony.
Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.
Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.