Difference between revisions of "A Brief Description of What We Will Do"
From Hackteria Wiki
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
'''[[Step 1 - Taking dry DNA from wells]]''' | '''[[Step 1 - Taking dry DNA from wells]]''' | ||
| − | |||
'''[[Step 2 - Transforming competent cells.]]''' | '''[[Step 2 - Transforming competent cells.]]''' | ||
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ||
| − | |||
'''Step 3 - Pick a single colony.''' | '''Step 3 - Pick a single colony.''' | ||
| Line 10: | Line 8: | ||
'''Step 4 - Inoculate broth with Ampicillin-R''' (an antibiotic) and let it grow for 18 hours. | '''Step 4 - Inoculate broth with Ampicillin-R''' (an antibiotic) and let it grow for 18 hours. | ||
| − | '''Step 5 - Use the resulting culture for mini-prep.''' - ''a process used to purify plasmids and yields clean, usable DNA.'' | + | '''Step 5 - Use the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' |
| + | |||
| + | '''Step 6 - [[Digesting the DNA]]''' | ||
| + | |||
| + | '''Step 7 - [[Purifying the DNA]]''' | ||
Latest revision as of 18:59, 16 June 2009
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Pick a single colony.
Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.
Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Purifying the DNA
