Difference between revisions of "A Brief Description of What We Will Do"

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'''Step 1 - Taking dry DNA from wells'''
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'''[[Step 1 - Taking dry DNA from wells]]'''
  
1. Making sure the plates are properly oriented, punch a hole through the foil cover using a pipette - into the well you want the DNA from. Don't remove foil cover since it might pose the threat of cross-contamination between wells.
 
 
2. Add 15uL of deionized water - diH2O
 
 
'''[[Step 2 - Transforming competent cells.]]'''
 
'''[[Step 2 - Transforming competent cells.]]'''
 
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.''
 
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.''
 
  
 
'''Step 3 - Pick a single colony.'''
 
'''Step 3 - Pick a single colony.'''
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'''Step 4 - Inoculate broth with Ampicillin-R''' (an antibiotic) and let it grow for 18 hours.
 
'''Step 4 - Inoculate broth with Ampicillin-R''' (an antibiotic) and let it grow for 18 hours.
  
'''Step 5 - Use the resulting culture for mini-prep.''' - ''a process used to purify plasmids and yields clean, usable DNA.''
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'''Step 5 - Use the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
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'''Step 6 - [[Digesting the DNA]]'''
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'''Step 7 - [[Purifying the DNA]]'''

Latest revision as of 17:59, 16 June 2009

Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.

Step 3 - Pick a single colony.

Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.

Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA

Step 7 - Purifying the DNA