Difference between revisions of "A Brief Description of What We Will Do"
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− | '''Step 1 - Taking dry DNA from wells''' | + | '''[[Step 1 - Taking dry DNA from wells]]''' |
− | + | '''[[Step 2 - Transforming competent cells.]]''' | |
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− | '''Step 2 - Transforming competent cells.''' | ||
''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ''Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.'' | ||
− | + | '''Step 3 - Pick a single colony.''' | |
− | + | '''Step 4 - Inoculate broth with Ampicillin-R''' (an antibiotic) and let it grow for 18 hours. | |
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− | + | '''Step 5 - Use the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' | |
− | + | '''Step 6 - [[Digesting the DNA]]''' | |
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− | '''Step | ||
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− | '''Step | + | '''Step 7 - [[Purifying the DNA]]''' |
Latest revision as of 18:59, 16 June 2009
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Pick a single colony.
Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.
Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA
Step 7 - Purifying the DNA