Difference between revisions of "A Brief Description of What We Will Do"
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'''Step 5 - Use the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' | '''Step 5 - Use the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.'' | ||
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+ | '''Step 6 - Digesting the DNA''' |
Revision as of 15:57, 16 June 2009
Step 1 - Taking dry DNA from wells
Step 2 - Transforming competent cells. Competent cells are those cells which have the ability to take up extracellular/naked DNA from its environment.
Step 3 - Pick a single colony.
Step 4 - Inoculate broth with Ampicillin-R (an antibiotic) and let it grow for 18 hours.
Step 5 - Use the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - Digesting the DNA