Bio Lab Infrastructure

How to start a DIWO art-sci bio lab?

If you can't build your lab, you don't own the lab

See a version from Marc Dusseiller aka dusjagr, Workshopologist and from Urs Gaudenz, Dipl. Ing. Mikrotechnik

See an old version of the slides

People not Infrastructure

• Lab Making | The Book: HackteriaLab 2014 - Yogyakarta, by Urs Gaudenz, Sachiko Hirosue

Instructions on lab-making

"Making a Laboratory is both a spontaneous activity and a slow labor of love."

A Lab is a place to labor (not only of love), experiment, research and share - there is no labor, experiment, research or sharing without the people. A Lab is not a museum for journalists, but a place made by a person for other people Start a small Lab , put it in a box, suitcase, backpack, picnic basket and take it with you. A Mobile Lab it actually allows you to go to people, and not wait for the others to come.

Now also on Medium, revised edition.

• Lab Infrastructure / Equipment

Experiences from setting up the BioTehna Lab Infrastructure. People not equipment. 20% Science 80% Rock'n'Roll.

Materials list for electronic prototyping from ali-express: here

Thoughts about Infrastructural Hospitality

People putting boxes to make people feel comfortable... like someone running a restaurant, make sure your people are well fed and feel comfortable.

Biosafety and other good practices

We have collected a lot of information about DIY-BSL1 and specificially looked at the regulations in Switzerland and how to use the DIY-BSL1 Manual Ecogen to register you lab for level 1 experiments using genetically modified microbes. Also specifically for RandeLab-GMP we have played through some of the concepts of "Gemütliche Mikrobiologische Praxis".

Infrastructure

BioLab Equipment

image	#	what	notes
	1	small hotplate
	1	Mixer
	1	small balance 0.02g - 1000g
	1	HotDog machine	it's like a small cooker/pot
	1	Coffee grinder	thanks bordul :-)
	1	DIY microscope PS3eye	made by tamberg, used by many
	2	DIY Microscopy kits	made by gaudi, Laser-cut microscopy stages
	1	DIY Spectroscope PS3eye	made by Bengt, check public laboratory
	1	scanner with embeddedincubator	made by bostjan et al at NanoSmano 3

BioLab stuff

image	#	what	notes
	4 x Erlenmayer	0 x beakers	1 x 100ml graded cylinder
	1	check online free samples or chinese stuff
	2	small cooking pots	we need a steampot for sterilization!
	var.	kitchen plastic ware	many tupperware
	1 x UV light bulp (black light)	1 x 254nm / 366nm tube	4 x UV-LED's
	1	DIY gel-box	var. Food colours	1 pack of Agar Agar	we need more agar!

General Technical Equipment

image	#	what	notes
	6	Soldering Irons	some of them with swiss plug...
	3	Table lamps	maybe get 3 more?
	1	Power Supply 15V, 2A	maybe get a stronger one, up to 80V
	1	Cordless Dremel and toolsl	belongs to dusjagr
	1	Dymo LetraTag	we need green ammo, from kniigica next to nama

Shared Tools for Hacking Mobile drawers

RAKO boxes labelled with Hackteria / Kapelica

image	Stuff	thing 1	thing 2	thing 3	thing 4	thing 5
	3	Table lamps	maybe get 3 more?
	5 x breadboards	2 x multimeters	crocodile cables	Jumper wires
	2 x large glue gun (+ammo)	1 x small glue gun (+ammo)	1 x epoxy	rubber bands	cable binders	get more small glue guns and loads of ammo
	9V blocks	30 AA Batteries	20 AAA Batteries	various power supplies 5 - 15V
Error creating thumbnail: File missing	4 x X-Mini active speakers	4-5 x random speakers	some cables
	5 x Helping hands	2 x Unsoldering tools	what else?

Electronics and Parts

RAKO boxes labelled with Hackteria / Kapelica

image	Class	Group 1	Group 2	Group 3	Group 4	Group 5
	Chips	10 attiny 85	20 4093	various
	4 x arduinos	Table lamps	get 10 large USB cables, and more arduinos
	Chips	10 attiny 85	20 4093	various
	2x	Mignon Gamekit	3 x Programmers	see info on [1]

Boxes

RAKO boxes labelled with Hackteria / Kapelica

image	content	content	content	Content	notes
	5 x Soldering Irons	1 x Solder wire	5 x sponges	anything else?
	2 x Table lamps	2 x Power Plugs	3 x 220V Timers	3 x remote control 220V Timers
	1	loads of fun stuff	see BioCyberKidzz

Wishlist

Lab Gear

- trash bin for separating garbage
- incubator
- fridge with freezer
- additional shelves
- DIY PCR
- couple of shelves
- centrifuge
- OD meter

Where to get stuff (in Ljubljana)

Organisms

http://lgcstandards-atcc.org/Default.aspx?base

http://www.ccap.ac.uk/

Chemicals

Slovenian distributors of lab chemicals, including enzymes and stuff

http://www.majbert.com/

http://www.kemomed.si/

http://www.omega.si/

http://www.kefo.si/

Everything you ever wanted to know about agar http://www.fao.org/docrep/X5822E/x5822e03.htm

Labware

http://www.tehtnica.si/

http://www.sanolabor.si/

http://market.mikro-polo.si/

http://dx.com/p/mg16129-c-5-led-auxiliary-clip-magnifier-black-white-eu-plug-110-240v-3-x-aa-193552

Electronics

http://www.hte.si/

http://www.conrad.si/ (supposedly too expensive)

http://dx.com/ order gadgets and electronics from china/HK

Helpful local institutions, companies and other people we have had pleasant interactions with in the past

http://www.algen.si/

http://ibk.mf.uni-lj.si/defaultslo.html

http://www.arhiv.mop.gov.si/si/o_ministrstvu/organiziranost/direktorat_za_okolje/

Other links on how to start a DIWO BioHackArt lab

From the Waag Do-It-Together biolab check their File:Waag wishlist.pdf

Using eBay to set up a molecular biology lab: costs less than $1000!

exerpts from the DIYbio maillist assembled on openWetWare

Instructable how to set up a home chemistry lab

makezine, setting up a home science lab part 1 part 2, part 3

From IndieBiotech aka Cathal Garvey not much on it, but a start.

more of Cathal's protocolls on github

Good Microbiological Practice

http://www.lbl.gov/ehs/biosafety/manual/html/AppxD.shtml