Hack-a-Taq, 6,7&13 April 2013
Swiss Meet-Up of the Hackteria network in Lausanne. Join us to discuss biohacking and DIY biology, help us setting up a new community bio-lab, have fun with self-made PCR thermocyclers and open-sourced Taq polymerases.
Contact: Sachiko
Objectives
1. test the Wild OpenPCR machine (gaudilabs)
2. hack the Taq isolation protocol from Open Biotechnology, and see if we can make and purify the enzyme with alternative reagents from the consumable reagents sold there (hackteria open source bioart…)
3. test out some PCR primers to detect E. coli (biodesign for the real world)
more on the wiki
Visits to EPFL labs
Preliminary Results
Testing the PCR thermocyclers
We tested different PCR thermocyclers, a commercial T3000 and the two homemade PCRs made by Urs. We tested a sample of e.coli using a primer for the tuf gene, distrubuted the sample into all the different thermocyclers and let it run. Tested the PCR product both in a DIY gelBox and a lab grade setup. Turns out the WildOpen PCRs work perfectly! (seems dusjagr messed up the pipetting and added the ladder to the samples that were in the t3000… call it an inline reference 🙂
pOpenTaq transfection
We cultured some Rosetta e.coli strain in the teaching labs of EPFL. Seems our first try went wrong due to the fact that we added the ampicillin antibiotics to early after the transfection, so the cells werent ready yet to cope with it. Then we did another try and got the cultures growing nicely. Added the IPTG after we reached a good density of the culture and let them produce the Taq… Havent tested the product yet.
Impressions
“Swiss Meet-Up of the Hackteria network in Lausanne. Join us to discuss biohacking and DIY biology, help us setting up a new community bio-lab, have fun with self-made PCR thermocyclers and open-sourced Taq polymerases.”
From Hack-a-Taq, Lausanne. Posted by Marc Dusseiller Dusjagr on 4/07/2013 (20 items)
- Getting started with the preps for Hack-a-Taq. 100ng of DNA sounds like more than it …
- Making dilutions of pOpenTaq to share with friends.
- Installing the biochemistry lab at HiroKouri
- Crisis at Hack-a-Taq, nothing grew….
- Kris helps troubleshooting the transfection protocoll
- Waiting for the bacteria to grow. Biology needs patience… and soft pillows!
- Ralf streaks some Rosettas. Never forget the labels 🙂
- Hack-a-Taq welcome party at EPFL….
- Visiting some labs at EPFL
- Checking what the peeps at EPFL look at with their microscopes
- Visit to EPFL for some Taq hacking. Nice sponsors they have at the RLC.
- Checking some regulations about the use of GMO in closed systems.
- For safety reason the lid of your centrifuge schould always be loaded with a heavy ob…
- Testing the different PCR Thermocyclers, the T3000, the WildOpen PCR and the new MyOp…
- Hmmmmm, loading these gels is not so easy….
- Testing the products of the MyPersonalOpenPCR. It’s looking good….
- Who needs a transilluminator anyway.
- Analysing our gel to compare the different PCR machines…
- Some image adjustments… YES! There definately is a line! And in all the PCR machine…
- Result from the IDEXX coliform detection after ca 40 hours of culture at random tempe…
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this will be amazing! super cool prototyping work, very advanced!
I am curious to see if all protocolls work out.
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