Preparing the pBAD and Lysis plasmids for gel electrophoresis

1. Take 1.5µl of the culture in an epenndorff tube.

1. Centrifuge the culture @ 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.

1. Repeat process to get required quantity of the cultures i.e. (1.5 µl twice)3.0 µl and centrifuge again.

1. The liquid around the collected pellet, called the supernatant can be discarded.

1. Dissolve the pellet in 150µl of ALS I previously stored at 4 degrees Celsius.

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