Difference between revisions of "Preparing the pBAD and Lysis plasmids for gel electrophoresis"
From Hackteria Wiki
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#Take 1.5µl of the culture in an epenndorff tube. | #Take 1.5µl of the culture in an epenndorff tube. | ||
| − | # Centrifuge the culture | + | |
| + | # Centrifuge the culture @ 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube. | ||
| + | |||
| + | #Repeat process to get required quantity of the cultures i.e. (1.5 µl twice)3.0 µl and centrifuge again. | ||
| + | |||
#The liquid around the collected pellet, called the supernatant can be discarded. | #The liquid around the collected pellet, called the supernatant can be discarded. | ||
| − | #Dissolve the pellet in 150µl of [[ALS I]] | + | |
| + | #Dissolve the pellet in 150µl of [[ALS I]] previously stored at 4 degrees Celsius. | ||
| + | |||
| + | |||
... | ... | ||
Revision as of 07:31, 12 June 2009
- Take 1.5µl of the culture in an epenndorff tube.
- Centrifuge the culture @ 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
- Repeat process to get required quantity of the cultures i.e. (1.5 µl twice)3.0 µl and centrifuge again.
- The liquid around the collected pellet, called the supernatant can be discarded.
- Dissolve the pellet in 150µl of ALS I previously stored at 4 degrees Celsius.
...
