Difference between revisions of "Preparing the pBAD and Lysis plasmids for gel electrophoresis"
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(Created page with '#Take 1.5ml of the culture in an epenndorff tube. # Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in th...') |
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# Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube. | # Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube. | ||
#The liquid around the collected pellet, called the supernatant can be discarded. | #The liquid around the collected pellet, called the supernatant can be discarded. | ||
− | #Dissolve the pellet in 150µl ALS I | + | #Dissolve the pellet in 150µl of[ALS I] |
+ | ... |
Revision as of 06:20, 9 June 2009
- Take 1.5ml of the culture in an epenndorff tube.
- Centrifuge the culture at 13200 rpm for 3 minutes, making sure the centrifuge is balanced. The centrifugal force created in the centrifuge forces the bacteria in the solution to collect in a pellet at the bottom of the eppendorff tube.
- The liquid around the collected pellet, called the supernatant can be discarded.
- Dissolve the pellet in 150µl of[ALS I]
...