Difference between revisions of "Guava seeds synthesized silver nanowires"

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m (→‎The synthesis of AgNWs with guava seed)
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[[File:AgNWs_made_with_guava_seeds.png|400px|thumb|right|diagram of how to synthesis AgNWs by the extraction of guava seed.]]
 
[[File:AgNWs_made_with_guava_seeds.png|400px|thumb|right|diagram of how to synthesis AgNWs by the extraction of guava seed.]]
  
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==The synthesis of AgNWs with guava seed==
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==The first try of Ag-NW bio synthesis in BioClub Tokyo==
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3 beakers of mixtures of silver nitrate and guava seed extraction were created with different concentration of guava seed extraction, 1. silver nitrate solution mixed with distilled water, 2. silver nitrate solution mixed with guava seed extraction heat stirred for 50 mins, 3. silver nitrate solution mixed with guava seed extraction heat stirred for 4 hours. All beakers are put under UV exposure for 4 hours, the color of the second and 3rd mixture were changed from milky white to orange. The black material floating on the final solution was collected by dropper and store in test tube.
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3 beakers of mixtures of silver nitrate and guava seed extraction were created with different concentration of guava seed extraction, 1. silver nitrate solution mixed with distilled water, 2. silver nitrate solution mixed with guava seed extraction heat stirred for 50 mins, 3. silver nitrate solution mixed with guava seed extraction heat stirred for 4 hours. All beakers were given UV exposure for 4 hours, the color of the second and 3rd mixture were changed from milky white to orange, it is filtered by filter paper and a final orange solution is collected. The final sample is centrifuged and the aggregations are also collected. Only the orange final solution was analyzed with UV-vis.
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#Preparation of the guava seed. The guava seed was collected from one Taiwanese guava by hand, then it is dried in an oven under 50ºC for 48 hours and powdered by a pharmacy pestle.
 
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#Mix 0.1g of the guava seed powder with 100ml distilled water, and it's stirred under 40ºC for 50 minutes. Filter out the seeds from the extraction solution.
 
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#Prepare 1mM silver nitrate solution by dissolving 1.7 grams of silver nitrate in 100 ml of distilled water.
 
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#Mix the silver nitrate solution and extraction of Taiwan guava seed by the ratio of 2:1.
 
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#Put the mixed solution under UV exposure for 4 hours, the beakers are sealed with cling film.
 
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#Collect the AgNWs solution. (yet to know how to collect it exactly)
 
  
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To collect the silver nanowires, you should focus on isolating the silver matter floating on the solution surface, as this is likely the nanowire component. Here's a step-by-step guide on how to collect the silver nanowires using a centrifuge and filter paper:
 
  
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#Centrifuge the solution: Transfer the entire reaction mixture, including the orange solution and the floating silver matter, to centrifuge tubes. Ensure that the tubes are suitable for centrifugation and can withstand the required speed.
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<gallery mode="traditionall" widths=400px>
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#Centrifugation: Place the centrifuge tubes into the centrifuge and run it at a suitable speed and time to separate the silver nanowires from the solution. The centrifugal force will cause the heavier silver nanowires to separate from the solution and collect at the bottom of the tubes.
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File:Seed of Taiwan guava.jpg|Preparation of the guava seed. The seeds was extracted from a Taiwanese guava.
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#Remove the supernatant: After centrifugation, carefully remove the tubes from the centrifuge. The centrifugation process should have caused the silver nanowires to form a pellet at the bottom of the tubes, while the orange solution (containing smaller nanoparticles) should be present as the supernatant liquid above the pellet.
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File:Powdered Taiwan guava seeds with pestle.jpg|The seeds were dried in oven at 50ºC for 48 hours and grinder with pharmacy grinder pestle.
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#Discard the supernatant: Gently pour off or aspirate the supernatant liquid containing the orange solution without disturbing the pellet of silver nanowires at the bottom. Be cautious not to disturb the nanowire pellet during this step.
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File:preparation of the extraction of Taiwan guava seed.jpeg|Mix 0.1g of the guava seed powder with 100ml distilled water, and it's stirred under 40ºC for 50 minutes. Filter out the seeds from the extraction solution.
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#Wash the nanowires: To remove any remaining contaminants or unreacted species, you can wash the silver nanowire pellet. Add a suitable solvent, such as ethanol or water, to the tubes and resuspend the pellet. Then, centrifuge again to separate the nanowires from the washing solvent. Repeat this washing process a couple of times to ensure proper purification.
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File:silver nitrate solution with guava extraction before UV.jpeg|Mix the silver nitrate solution and extraction of Taiwan guava seed by the ratio of 2:1.
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#Dry the nanowires: After washing, you can either air-dry the nanowires or use a gentle heat source to evaporate the solvent and dry the nanowires. It's essential to be gentle during the drying process to maintain the integrity of the nanowires.
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File:silver nitrate solution with guava extraction during UV.jpeg|Put the mixed solution under UV exposure for 4 hours, the beakers are sealed with cling film.
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#Collect the dried nanowires: Once dried, carefully remove the silver nanowires from the centrifuge tubes using a spatula or another suitable tool. You now have the purified silver nanowires ready for further analysis or application.
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File:silver nitrate solution with guava extraction after UV.jpeg|After UV exposure, the 2nd and 3rd solutions appear orange.
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<gallery mode="traditional" widths=200px>
 
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File:Seed of Taiwan guava.jpg|Seed of Taiwan guava
 
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File:Powdered Taiwan guava seeds with pestle.jpg|Powdered Taiwan guava seeds with pestle
 
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File:preparation of the extraction of Taiwan guava seed.jpeg|preparation of the extraction of Taiwan guava seed
 
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File:silver nitrate solution with guava extraction before UV.jpeg|silver nitrate solution with guava extraction before UV
 
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File:silver nitrate solution with guava extraction during UV.jpeg|silver nitrate solution with guava extraction during UV
 
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File:silver nitrate solution with guava extraction after UV.jpeg|silver nitrate solution with guava extraction after UV
 
 
File:final solution of AgNWs synthesis with Taiwan guava seeds.jpeg|final solution of AgNWs synthesis with Taiwan guava seeds
 
File:final solution of AgNWs synthesis with Taiwan guava seeds.jpeg|final solution of AgNWs synthesis with Taiwan guava seeds
 
</gallery>
 
</gallery>
  
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====UV-vis analysis of AgNWs====
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====Analyzing result====
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The [https://en.wikipedia.org/wiki/Ultraviolet%E2%80%93visible_spectroscopy Ultraviolet–visible spectroscopy] test was supported by Shohei Asami, member of Bioclub, he's currently in his doctoral studies. He had the sample testified by the machine in his company laboratory. UV-Vis spectra of Ag-NWs synthesis with Taiwan guava seeds, [http://https://docs.google.com/spreadsheets/d/1UpdfWLr9N90NuxQ16fV9wc6cabsoONLBazMyCx_sYj0/edit?usp=sharing data]is provided by Shohei Asami. The result match up the graph in the paper, but it's overall lower, it might due to its lower density.
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UV-vis the final orange solution. The [https://en.wikipedia.org/wiki/Ultraviolet%E2%80%93visible_spectroscopy Ultraviolet–visible spectroscopy] test was made by Shohei Asami, member of Bioclub. The final result can be found  [http://https://docs.google.com/spreadsheets/d/1UpdfWLr9N90NuxQ16fV9wc6cabsoONLBazMyCx_sYj0/edit?usp=sharing here]. The curve match up the graph in the paper but overall lower, indicates the low density of silver nano particles.
  
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<gallery mode="traditionall" class="center" heights=200px caption="UV-vis result of the final solution>
 
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File:Shohei Asami.jpg|UV-vis graphs provider: Shohei Asami
 
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File:orange AgNWs and clear one2.png|UV-Vis spectra of the final solution synthesised with Taiwan guava seeds
 
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File:UV-Vis spectra of P. Guajava extract and Ag-NWs.png|UV-Vis spectra of P. Guajava extraction and the final solution mentioned in the paper
 
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</gallery>
 
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====SEM result====
 
 
The scanning electron microscopy is kindly supported by Joakim Reuteler in ETH Zurich. 2 samples of guava seeds synthesised AgNWs were prepared, one is AgNW solution dried on glass, the other one is aggregation collected from the solution. However we didn't find any wire-alike shape, indicating the failure of the nano wire synthesis.  
 
The scanning electron microscopy is kindly supported by Joakim Reuteler in ETH Zurich. 2 samples of guava seeds synthesised AgNWs were prepared, one is AgNW solution dried on glass, the other one is aggregation collected from the solution. However we didn't find any wire-alike shape, indicating the failure of the nano wire synthesis.  
  
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<gallery mode="traditional" class="center" heights=200px caption="SEM images of the final solutions">
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<gallery mode="traditional" class="center" heights=200px caption="SEM and UV-vis of the final solutions">
 
File:low-density-Ag-NW_12.jpeg|SEM image of the final solution in 10um
 
File:low-density-Ag-NW_12.jpeg|SEM image of the final solution in 10um
 
File:low-density-Ag-NW_11.jpeg|SEM image of the final solution in 40um
 
File:low-density-Ag-NW_11.jpeg|SEM image of the final solution in 40um
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File:Shohei Asami.jpg|UV-vis graphs provider: Shohei Asami
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File:orange AgNWs and clear one2.png|UV-Vis spectra of the final solution synthesised with Taiwan guava seeds
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File:UV-Vis spectra of P. Guajava extract and Ag-NWs.png|UV-Vis spectra of P. Guajava extraction and the final solution mentioned in the paper
 
</gallery>
 
</gallery>
  

Revision as of 13:14, 21 October 2023

Part I, the bio synthesis of Ag-NWs

This experiment is composed with two existed paper, one is to synthesis AgNWs with Psidium Guajava seed, and the other one is to synthesis transparent conductive layer by transferring AgNWs which are light sintered by Riso print Gocco lamps. Guava seeds contain some compounds, such as polyphenols and alkaloids, which can reduce silver nitrate under certain conditions, thereby promoting the growth and stability of silver nanowires. These compounds acted as reducing agents and stabilizers during the preparation process, thus enabling a green synthesis method without external stabilizers. For the light sintering of AgNWs, a local Japanese toy kit "RISO PRINT GOCCO LAMP" is used to replace the IPL (intense pulsed light) in the original paper. The sintered AgNWs is formed on glass and then be transferred to a PVB film.

diagram of how to synthesis AgNWs by the extraction of guava seed.

The first try of Ag-NW bio synthesis in BioClub Tokyo

3 beakers of mixtures of silver nitrate and guava seed extraction were created with different concentration of guava seed extraction, 1. silver nitrate solution mixed with distilled water, 2. silver nitrate solution mixed with guava seed extraction heat stirred for 50 mins, 3. silver nitrate solution mixed with guava seed extraction heat stirred for 4 hours. All beakers were given UV exposure for 4 hours, the color of the second and 3rd mixture were changed from milky white to orange, it is filtered by filter paper and a final orange solution is collected. The final sample is centrifuged and the aggregations are also collected. Only the orange final solution was analyzed with UV-vis.


  • Preparation of the guava seed. The seeds was extracted from a Taiwanese guava.

  • The seeds were dried in oven at 50ºC for 48 hours and grinder with pharmacy grinder pestle.

  • Mix 0.1g of the guava seed powder with 100ml distilled water, and it's stirred under 40ºC for 50 minutes. Filter out the seeds from the extraction solution.

  • Mix the silver nitrate solution and extraction of Taiwan guava seed by the ratio of 2:1.

  • Put the mixed solution under UV exposure for 4 hours, the beakers are sealed with cling film.

  • After UV exposure, the 2nd and 3rd solutions appear orange.

  • final solution of AgNWs synthesis with Taiwan guava seeds

Analyzing result

UV-vis the final orange solution. The Ultraviolet–visible spectroscopy test was made by Shohei Asami, member of Bioclub. The final result can be found here. The curve match up the graph in the paper but overall lower, indicates the low density of silver nano particles.

The scanning electron microscopy is kindly supported by Joakim Reuteler in ETH Zurich. 2 samples of guava seeds synthesised AgNWs were prepared, one is AgNW solution dried on glass, the other one is aggregation collected from the solution. However we didn't find any wire-alike shape, indicating the failure of the nano wire synthesis.

  • SEM and UV-vis of the final solutions

  • SEM image of the final solution in 10um

  • SEM image of the final solution in 40um

  • UV-vis graphs provider: Shohei Asami

  • UV-Vis spectra of the final solution synthesised with Taiwan guava seeds

  • UV-Vis spectra of P. Guajava extraction and the final solution mentioned in the paper

Part II, Gacco Lamp Sintered AgNWs PVM film

Continued with the first part, in the original paper, the AgNWs is sintered by IPL (intense pulsed light) but due to the high price of IPL, I decided to try something else. I found a type of light bulb that generate sudden high heat in a Japanese toy kit RISO PRINT GOCCO LAMP which is introduced by the founder of Bioclub, Georg Tremmel. The original purpose of the bulb is to create mask for riso print. The original usage please visit RISO PRINT GOCCO LAMP tutorial. The follow on practices is documented in page Conductive thin coating made of AgNWs.

diagram of how to synthesis AgNWs by the extraction of guava seed.

A conductive, uniform, and ultra-smooth flexible transparent composite film is produced by embedding silver nanowires (AgNWs) into poly(vinyl-butyral) (PVB) without pressure or high-temperature annealing.

  • RISO PRINT GACCO lamp

  • close-up photo of the GACCO lamp

  • bio club open lab day

Preferences

  1. Ali, Faisal, Zahid Ali, Umer Younas, Awais Ahmad, Ghulam Mooin Ud Din, Muhammad Pervaiz, Rafael Luque, et al. 2021. “UV-Light Mediated Biosynthesis of Silver Nanowires; Characterization, Dye Degradation Potential and Kinetic Studies.” Sustainability 13 (November): 13220. https://doi.org/10.3390/su132313220.
  2. Lv, Pengfei, Huimin Zhou, Min Zhao, Dawei Li, Keyu Lu, Di Wang, Jieyu Huang, Yibing Cai, Lucian Amerigo Lucia, and Qufu Wei. 2018. “Highly Flexible, Transparent, and Conductive Silver Nanowire-Attached Bacterial Cellulose Conductors.” Cellulose 25 (6): 3189–96. https://doi.org/10.1007/s10570-018-1773-8.
  3. Ferraro, Giovanni, and Emiliano Fratini. 2019. “A Simple Synthetic Approach To Prepare Silver Elongated Nanostructures: From Nanorods to Nanowires.” Journal of Chemical Education 96 (3): 553–57. https://doi.org/10.1021/acs.jchemed.8b00628.
  4. Padhi, Santwana, and Anindita Behera. 2022. “Chapter 17 - Biosynthesis of Silver Nanoparticles: Synthesis, Mechanism, and Characterization.” In Agri-Waste and Microbes for Production of Sustainable Nanomaterials, edited by Kamel A. Abd-Elsalam, Rajiv Periakaruppan, and S. Rajeshkumar, 397–440. Nanobiotechnology for Plant Protection. Elsevier. https://doi.org/10.1016/B978-0-12-823575-1.00008-1.
  5. Lee, Dong Jun, Youngsu Oh, Jae-Min Hong, Young Wook Park, and Byeong-Kwon Ju. 2018. “Light Sintering of Ultra-Smooth and Robust Silver Nanowire Networks Embedded in Poly(Vinyl-Butyral) for Flexible OLED.” Scientific Reports 8 (1): 14170. https://doi.org/10.1038/s41598-018-32590-0.
  6. Jin, Hwa-Young, Jae-Yup Kim, Jin Ah Lee, Kwangsoo Lee, Kicheon Yoo, Doh-Kwon Lee, BongSoo Kim, et al. 2014. “Rapid Sintering of TiO2 Photoelectrodes Using Intense Pulsed White Light for Flexible Dye-Sensitized Solar Cells.” Applied Physics Letters 104 (14): 143902. https://doi.org/10.1063/1.4871370.
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