Difference between revisions of "GlobalLAMP-Paris"
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=== Results === | === Results === | ||
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+ | ''' Devices designs ''' | ||
+ | Chili qLAMP -> Comming soon! <br> | ||
+ | WebCam qLAMP -> Comming soon! <br> | ||
+ | Open qLAMP -> [https://gitlab.com/open-bioeconomy-lab/diagnostics-hardware/rt-lamp-device/-/tree/master/open_qLAMP] | ||
+ | |||
+ | ''' Prototypes benchmark ''' | ||
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At the end of the workshop we compared the ability of the three prototypes (webcam qLAMP, chili qLAMP and [https://gitlab.com/open-bioeconomy-lab/diagnostics-hardware/rt-lamp-device/-/tree/master/open_qLAMP open qLAMP]) to amplify 200 SARS-CoV-2 inactivated viral units using the [https://pubmed.ncbi.nlm.nih.gov/35027866/ CoronaDetective] protocol in the green channel and the [GMO detective https://gmodetective.com/] in the red channel. The GMO should generate less signal as the filters we're using are for green fluorescence, which filters the vast majority of the red signal. | At the end of the workshop we compared the ability of the three prototypes (webcam qLAMP, chili qLAMP and [https://gitlab.com/open-bioeconomy-lab/diagnostics-hardware/rt-lamp-device/-/tree/master/open_qLAMP open qLAMP]) to amplify 200 SARS-CoV-2 inactivated viral units using the [https://pubmed.ncbi.nlm.nih.gov/35027866/ CoronaDetective] protocol in the green channel and the [GMO detective https://gmodetective.com/] in the red channel. The GMO should generate less signal as the filters we're using are for green fluorescence, which filters the vast majority of the red signal. | ||
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Revision as of 12:15, 23 June 2022
Collaborative Developement of Isothermal Amplification & Devices
June 11. - 19, 2022 Paris
Scope of the Project
Quantitative real-time DNA amplification is at the heart of either diagnosing infectious disease or any section of biology research. Use of these devices has been limited due its cost and availability, therefore, refuting to elaborate and end-point methods like gel-electrophoresis. This project will change accessibility and affordability of this device to specially low-income countries either for POC or teaching purposes. Furthermore, the core of the project includes researchers and collaborators from diverse geographical backgrounds, professional expertise and socioeconomic origins, either at the development stage or at the end-user level at the next phases.
[Project Application [1]]
Participants
- Francisco Javier Quero (France)
- Urs Gaudenz (Switzerland)
- Yanwu Guo (Oslo)
- Fernán Federici Noe (Chile)
- Boris Enrique Orstica Navarrete (Chile)
- Harry Akligoh (Ghana)
- Ganga Chinna Rao Devarapu (remote)
- Smitha S Hegde (remote)
- Marc Dusseiller (remote / Indonesia)
- Shingo Hisakawa (remote Japan)
Programm
Saturday, June 11th 2022
● Visiting the different labs
● Bringing materials to the lab
● Taking over the house
● Welcome beers
● Lot's of bicycle riding
Sunday, June 12th
● chilling in the house
● defining program for the week
● tourist tour
● prepare kick-off presentation
● dinner
Monday, June 13th – Sharing Knowledge
● Kick-Off presentation
Setting up the lab
● Sensor Lab
● Lyophilization Intro
● Show and tell with prototypes
Tuesday, June 14th – Work Sessions
● Lab work session
● Hands On Workshops
● Amplification benchmark
Wednesday, June 15th – Networking
● Visiting local Hackerspaces / Fablabs
● Broadcasting from the lab
● Connecting with other GOSH communities
● Exchange with potential production partners
Thursday, June 16th – Design Sprints
● Rapid Prototyping
● Design
● Visualizations
Friday, June 17th – Future Outlook
● Assessment of achievements
● Minimal viable products
● Global potential analysis
● Potential collaboration partners
● Planing for phase 2
● Open Houses Vincennes
Saturday, June 18th – Guests & Celebrities
More to come:
Social Events
One day of lectures
Invite and connect to other GOSH communities
Public / broadcasted event / time for broadcast in the lab
Seeed and some of the production companies?
- Future outlook session (where do we want to go with our project / applications / specifications)
- Field trip (going out to nature) to test devices / protocols in the field
Prototypes:
- Induction heating - PocketPCR / Pocket qPCR - open qLAMP - Webcam PCR - GMO/Corona Detective - ChilloutLAMP -
- Program for the week - How to collaborate in the future - Say hello with Marc (Indonesia) and Shingo (Japan)
Locations
The Lab:
CRI (Center for Research and Interdisciplinarity)
CRI is co-constructing and sharing new ways of learning, teaching, conducting research and mobilizing collective intelligence in the fields of life, learning and digital sciences, in order to address the UN’s sustainable development goals (SDGs).
Address: Learning Planet Institute, 8bis rue Charles V, 75004, Paris.
Our Home-Stay:
House in the city with backyard, hosted by Alexandra
https://www.google.com/maps?daddr=48.849639892578125,2.443648099899292
49 Rue de la Liberté, Vincennes, Île-de-France 94300, France
Results
Devices designs
Chili qLAMP -> Comming soon!
WebCam qLAMP -> Comming soon!
Open qLAMP -> [2]
Prototypes benchmark
At the end of the workshop we compared the ability of the three prototypes (webcam qLAMP, chili qLAMP and open qLAMP) to amplify 200 SARS-CoV-2 inactivated viral units using the CoronaDetective protocol in the green channel and the [GMO detective https://gmodetective.com/] in the red channel. The GMO should generate less signal as the filters we're using are for green fluorescence, which filters the vast majority of the red signal.
WebCam qLAMP | Chili qLAMP | Open qLAMP |
---|---|---|
Event Funded by:
- GOSH Collaborative Development Program
- Shuttleworth Foundation Flash Grants
- Tsinghua University, Materials sponsor
- CRI, Center for Research and Interdisciplinarity
Thank you to our sponsors