Difference between revisions of "Fluorescence Optical Detector Board"
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PE-Streptavidin: <br> | PE-Streptavidin: <br> | ||
| − | Excitation: 488 blue (azure) <br> | + | Excitation: 488 nm blue (azure) (broad excitation, one peak 488 nm, one at 575 nm) <br> |
| − | Emission: 575 (yellow) <br> | + | Emission: 575 nm (yellow) <br> |
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Beads: <br> | Beads: <br> | ||
Revision as of 21:27, 10 September 2020
Learning from reverse engineering the Illumina Optical Detector Board extracted from a NeoPrep systen.
Luminex
Testing the sensor with "R&D Systems ® Luminex ® Bead-Based Assays for Multiplexing" kits.
There are two light sources used to collect data from samples. The red light source (laser for Luminex® 100/200™ and LED for MAGPIX®) excites the internal dyes in the microspheres so that they can be classified on the bead map. The green light source excites any reporter bound to the surface of the beads (PE-Streptavidin).
In Luminex applications, SA-PE is excited by the 532 nm green laser or 520 nm green LED.
Its emission peak exists at 575 ± 10 nm (orange/yellow).
Systems with two LEDs:
(511 nm) green
(621 nm) red
PE-Streptavidin:
Excitation: 488 nm blue (azure) (broad excitation, one peak 488 nm, one at 575 nm)
Emission: 575 nm (yellow)
Beads:
Excitation 621 nm red ?
Emission? two dyes for fluorescence? Infrared?
Links:
https://www.biolegend.com/en-us/products/pe-streptavidin-1475
